NDLI: Regulatory calcium effect on adenylyl cyclase functional activity in the infusorian Dileptis anser

Content Provider	Springer Nature Link
Author	Shpakov, A. O. Uspenskaya, Z. I. Derkach, K. V. Kuznetsova, L. A. Plesneva, S. A. Pertseva, M. N.
Copyright Year	2007
Abstract	Earlier we have shown that some non-hormonal activators of adenylyl cyclase (AC) and hormones of higher vertebrate animals are able to affect functional activity of the AC system in the infusorian Dileptus anser. In the present work, sensitivity of this infusorian AC to Ca$^{2+}$ was studied and it was found that calcium cations at concentrations of 0.5–10 μM stimulated significantly the enzyme activity in D. anser partially purified membranes. An increase of Ca$^{2+}$ concentrations to 100 μM and higher led to the complete block of their stimulatory effect. In the EDTA-treated membranes the enzyme activity was reduced markedly, but it was restored significantly by addition of Ca$^{2+}$. Calmodulin antagonists—chlorpromazine, W-7, and W-5—caused a dose-dependent decrease of the enzyme activity stimulated by 5 μM Ca$^{2+}$ with IC$_{50}$ values of 35, 137, and 174 M, respectively. The AC-stimulating effects of biogenic amines (serotonin and octopamine) were completely retained in the presence of 2.5 and 100 μM Ca$^{2+}$, whereas effects of peptide hormones (relaxine and EGF) were hardly changed in the presence of 2.5 μM calcium ions, but were markedly inhibited by 100 μM Ca$^{2+}$. In the EDTA-treated membranes, the AC effects of biogenic amines were reduced, while the effects of peptide hormones were not revealed. On addition of Ca$^{2+}$, the AC effects of biogenic amines were completely restored, whereas the effects of peptide hormones were not detected or restored to a non-significant degree. Calmodulin antagonists slightly affected the AC effects of peptide hormones at concentrations efficient in the case of vertebrate AC, but decreased them markedly at higher concentrations. The AC effects of biogenic amines were little sensitive even to high antagonist concentrations. The obtained data show that targets of action of peptide hormones in the infusorian D. anser cell culture are the AC forms whose activity depends on calcium cations and possibly is regulated by Ca$^{2+}$/calmodulin, whereas targets of action of biogenic amines are calcium-independent enzyme forms.
Starting Page	145
Ending Page	153
Page Count	9
File Format	PDF
ISSN	00220930
Journal	Journal of Evolutionary Biochemistry and Physiology
Volume Number	43
Issue Number	2
e-ISSN	16083202
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2007-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	adenylyl cyclase adenylyl cyclase signal system infusorian calmodulin calcium lower eukaryotes Dileptus anser Evolutionary Biology Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Biochemistry Ecology, Evolution, Behavior and Systematics

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