NDLI: Molecular Cloning and in Situ Expression Patterns of Two New Pheromone-Binding Proteins from the Corn Stemborer Sesamia nonagrioides

Content Provider	Springer Nature Link
Author	Santis, Federica François, Marie Christine Merlin, Christine Pelletier, Julien Maïbèche Coisné, Martine Conti, Eric Jacquin Joly, Emmanuelle
Copyright Year	2006
Abstract	We describe the identification and characterization of two new cDNAs encoding pheromone-binding proteins (PBPs) from the male antennae of Sesamia nonagrioides, a species where no PBPs have been identified to date. Because PBPs are thought to participate in the first step of odor detection in a specific manner, we focused our investigation on this olfactory protein family using reverse transcription–polymerase chain reaction strategies. The deduced amino acid sequences of SnonPBP1 and SnonPBP2 revealed mature proteins of 142 and 143 amino acids, respectively, with six cysteine residues in conserved positions relative to other known PBPs. The alignment of the two mature S. nonagrioides PBPs with other noctuid PBPs showed high sequence identity (70–80%) with other full-length sequences from GenBank. Sequence identity between SnonPBP1 and SnonPBP2 was only 46%, suggesting that the two proteins belong to different classes of PBPs already described from the Noctuidae. Furthermore, analyses of expression patterns of SnonPBP1 and SnonPBP2 were performed by in situ hybridization on antennae of both sexes, and these studies revealed the expression of the two PBPs at the bases of olfactory sensilla (basiconica or trichodea) from both sexes. The possible binding properties of these two new PBPs are discussed according to their homologies with other known PBPs and S. nonagrioides pheromone components.
Starting Page	1703
Ending Page	1717
Page Count	15
File Format	PDF
ISSN	00980331
Journal	Journal of Chemical Ecology
Volume Number	32
Issue Number	8
e-ISSN	15731561
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-08-02
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sesamia nonagrioides Noctuidae (Z)-11-hexadecenyl acetate Pheromone-binding proteins Molecular cloning In situ hybridization RT-PCR Stemborer Biological Microscopy Ecology Agriculture Physical Chemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Ecology, Evolution, Behavior and Systematics

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