NDLI: Two Regulatory Mechanisms of Monoterpenoid Pheromone Production in Ips spp. Of Bark Beetles

Content Provider	Springer Nature Link
Author	Bearfield, Jeremy C. Henry, Anastasia G. Tittiger, Claus Blomquist, Gary J. Ginzel, Matthew D.
Copyright Year	2009
Abstract	Bark beetles use aggregation pheromones to coordinate host colonization and mating. These monoterpenoid chemical signals are produced de novo in midgut cells via the mevalonate pathway, and pheromone production is induced when an adult beetle feeds on phloem of a host tree. In Ips pini, juvenile hormone (JH) III influences key regulatory enzymes along the mevalonate pathway that leads to pheromone production. In fact, topically applied JH III is sufficient to stimulate pheromone production in unfed males. In this study, we explore the influence of feeding and JH III treatment on pheromone production in male Ips confusus, the pinyon Ips. We also characterize the influence of feeding and JH III treatment on transcript levels and activity of three key enzymes involved in pheromone biosynthesis: 3-hydroxy-3-methylglutaryl-CoA (HMG) synthase (HMGS), HMG-CoA reductase (HMGR) and geranyl diphosphate synthase (GPPS). We also extend the current understanding of the regulation of pheromone biosynthesis in I. pini, by measuring the influence of feeding and JHIII treatment on enzymatic activity of HMGS and GPPS. Feeding on host phloem alone strongly induces pheromone production in male I. confusus, while JH III treatment has no effect. However, feeding and JH III both significantly up-regulate mRNA levels of key mevalonate pathway genes. Feeding up-regulates these genes to a maximum at 32 h, whereas with JH III-treatment, they are up-regulated at 4, 8, and 16 h, but return near to non-treatment levels at 32 h. Feeding, but not JH III treatment, also increases the activity of all three enzymes in I. confusus, while both feeding or treatment with JH III increase HMGS and GPPS activity in I. pini. Our data suggest that pheromone production in Ips is not uniformly controlled by JH III and feeding may stimulate the release of some other regulatory factor, perhaps a brain hormone, required for pheromone production.
Starting Page	689
Ending Page	697
Page Count	9
File Format	PDF
ISSN	00980331
Journal	Journal of Chemical Ecology
Volume Number	35
Issue Number	6
e-ISSN	15731561
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-06-25
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ips confusus Ips pini Pheromones Juvenile hormone Mevalonate pathway Agriculture Biological Microscopy Entomology Biochemistry Ecology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Ecology, Evolution, Behavior and Systematics

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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