NDLI: Response Surface Methodology Optimization of Melanin Production by Streptomyces cyaneus and Synthesis of Copper Oxide Nanoparticles Using Gamma Radiation

Content Provider	Springer Nature Link
Author	El Batal, Ahmed Ibrahim El Sayyad, Gharieb Saied El Ghamery, Abbas Gobara, Mohamed
Copyright Year	2016
Abstract	Melanin pigment is witnessed to be widely used in medicine, food, cosmetic preparations with strong hydrophobicity. Streptomyces cyaneus is utilized for the synthesis of melanin by optimizing culture conditions through screening of critical factors, by Response Surface Methodology as Plackett–Burman design (P-BD), while further statistical optimization was applied using Central Composite Design (CCD) for maximizing yield. Moreover, the impacts of gamma radiation and alternative frugal l-tyrosine, natural sources were studied as it predicted for remarkable rising in the pigment concentration. Herein unprecedented achievement was realized for melanin pigment production, (9.898 mg/ml) was obtaining by optimized culture condition. Also, 2.0% fava bean’s seed peel maximized melanin (9.953 mg/ml) and hence super-yield (11.113 mg/ml) was produced by a stimulus from gamma irradiation (2.5 kGy). The extracted melanin was confirmed chemically, UV–Vis, and FT-IR analysis. Copper oxide nanoparticles (CuONPs) were synthesized by radiolytic reactions of gamma radiation (30.0 kGy) in the presence of melanin as a capping agent. CuONPs were characterized by UV–Vis, DLS, XRD, and FT-IR. TEM analysis revealed the morphology of monodispersed CuONPs with a mean diameter 29.82 nm. CuONPs exhibited excellent antimicrobial activity against food born Gram-positive, Gram-negative bacteria, and fungi; they can find potential applications for the food packaging approach.
Starting Page	1083
Ending Page	1112
Page Count	30
File Format	PDF
ISSN	10407278
Journal	Journal of Cluster Science
Volume Number	28
Issue Number	3
e-ISSN	15728862
Language	English
Publisher	Springer US
Publisher Date	2016-10-25
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Melanin pigment Streptomyces cyaneus Response Surface Methodology Fava bean’s seed peel Gamma radiation Copper oxide nanoparticles Antimicrobial activity Catalysis Inorganic Chemistry Physical Chemistry Nanochemistry
Content Type	Text
Resource Type	Article
Subject	Chemistry Biochemistry Condensed Matter Physics Materials Science

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