NDLI: Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes

Content Provider	Springer Nature Link
Author	Lisowska, Katarzyna A. Dębska Ślizień, Alicja Jasiulewicz, Aleksandra Heleniak, Zbigniew Bryl, Ewa Witkowski, Jacek M.
Copyright Year	2011
Abstract	CD4$^{+}$ T lymphocytes of patients with chronic kidney disease (CKD) are characterized by reduced levels of crucial surface antigens and changes in the cell cycle parameters. Recombinant human erythropoietin (rhEPO) normalizes their altered phenotype and proliferative capacity. Mechanisms leading to the deficient responses of T lymphocytes are still not clear but it is postulated that immunological changes are deepened by hemodialysis (HD). Study of activation parameters of CD4$^{+}$ T lymphocytes in hemodialyzed and predialysis CKD patients could bring insight into this problem. Two groups of patients, treated conservatively (predialysis, PD) and hemodialyzed (HD), as well as healthy controls, were included into the study; neither had received rhEPO. Proportions of main CD4$^{+}$CD28$^{+}$, CD4$^{+}$CD25$^{+}$, CD4$^{+}$CD69$^{+}$, CD4$^{+}$CD95$^{+}$, and CD4$^{+}$HLA-DR$^{+}$ lymphocyte subpopulations and proliferation kinetic parameters were measured with flow cytometry, both ex vivo and in vitro. No differences were seen in the proportions of main CD4$^{+}$ lymphocyte subpopulations (CD4$^{+}$CD28$^{+}$, CD4$^{+}$CD25$^{+}$, CD4$^{+}$HLA-DR$^{+}$, CD4$^{+}$CD69$^{+}$, CD4$^{+}$CD95$^{+}$) between all examined groups ex vivo. CD4$^{+}$ T lymphocytes of HD patients exhibited significantly decreased expression of co-stimulatory molecule CD28 and activation markers CD25 and CD69 after stimulation in vitro when compared with PD patients and healthy controls. HD patients showed also decreased percentage of CD4$^{+}$CD28$^{+}$ lymphocytes proliferating in vitro; these cells presented decreased numbers of finished divisions after 72 h of stimulation in vitro and had longer G0→G1 time when compared to healthy controls. CD4$^{+}$ T lymphocytes of PD patients and healthy controls were characterized by similar cell cycle parameters. Our study shows that repeated hemodialysis procedure influences phenotype and proliferation parameters of CD4$^{+}$ T lymphocytes.
Starting Page	189
Ending Page	200
Page Count	12
File Format	PDF
ISSN	02719142
Journal	Journal of Clinical Immunology
Volume Number	32
Issue Number	1
e-ISSN	15732592
Language	English
Publisher	Springer US
Publisher Date	2011-10-13
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	CD4$^{+}$ T lymphocytes antigens proliferation hemodialysis Medical Microbiology Internal Medicine Infectious Diseases Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes

Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes

Effects of Exogenous IL-2 Administration on the Homeostasis of CD4+ T Lymphocytes

CD4$^{+}$ T Cells Downregulate Bcl-2 in Germinal Centers

Bmi-1 Regulates Autoreactive CD4$^{+}$ T Cell Survival in Immune Thrombocytopenia Patients

CD4$^{+}$CD7$^{−}$ T Cells: A Separate Subpopulation of Memory T Cells?

Maturation of CD4$^{+}$ Regulatory T Lymphocytes and of Cytokine Secretions in Infants Born Prematurely

Oligoclonal Expansion of CD45RO$^{+}$ T Lymphocytes in Omenn Syndrome

CD28–Negative CD8–Positive Cytotoxic T Lymphocytes Mediate Hepatocellular Damage in Hepatitis C Virus Infection

Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes

Similar Documents

Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes

Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes

Effects of Exogenous IL-2 Administration on the Homeostasis of CD4+ T Lymphocytes

CD4$^{+}$ T Cells Downregulate Bcl-2 in Germinal Centers

Bmi-1 Regulates Autoreactive CD4$^{+}$ T Cell Survival in Immune Thrombocytopenia Patients

CD4$^{+}$CD7$^{−}$ T Cells: A Separate Subpopulation of Memory T Cells?

Maturation of CD4$^{+}$ Regulatory T Lymphocytes and of Cytokine Secretions in Infants Born Prematurely

Oligoclonal Expansion of CD45RO$^{+}$ T Lymphocytes in Omenn Syndrome

CD28–Negative CD8–Positive Cytotoxic T Lymphocytes Mediate Hepatocellular Damage in Hepatitis C Virus Infection

Hemodialysis Affects Phenotype and Proliferation of CD4-Positive T Lymphocytes