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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ohki, Kaori Taniuchi, Yukiko |
| Copyright Year | 2009 |
| Abstract | The intercellular distribution of nitrogenase was detected in a natural population of Trichodesmium spp. collected from Shitaba Bay, Uwajima, Ehime Prefecture, Japan. Polyclonal antibodies raised against the Fe-protein and the MoFe-protein (α-subunit) of nitrogenase were used as probes. Visualization of the antibody binding to the nitrogenase was performed using horseradish peroxidase-conjugated secondary antibody and the chromogenic substrate, 3,3′-diaminobenzidine tetrahydrochloride, to avoid the masking effects caused by the bright autofluorescence emitted by the Trichodesmium cells. Nitrogenase proteins were detected in 66 to 81% of the trichomes. More than 77% of the cells were immunostained in individual nitrogenase-containing trichome. The data show natural populations of Trichodesmium spp. do not developed heterocyst-equivalent cells. |
| Starting Page | 427 |
| Ending Page | 432 |
| Page Count | 6 |
| File Format | |
| ISSN | 09168370 |
| Journal | Journal of Oceanography |
| Volume Number | 65 |
| Issue Number | 3 |
| e-ISSN | 1573868X |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-04-26 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Natural population of Trichodesmium spp. nitrogenase synthesis whole-cell immunolocalization diazotrophic cyanobacteria Freshwater & Marine Ecology Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oceanography |
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