NDLI: Impaired mitochondrial functions contribute to 3-bromopyruvate toxicity in primary rat and mouse hepatocytes

Content Provider	Springer Nature Link
Author	Sobotka, Ondřej Endlicher, René Drahota, Zdeněk Kučera, Otto Rychtrmoc, David Raad, Marjan Hakeem, Khurum Červinková, Zuzana
Copyright Year	2016
Abstract	A compound with promising anticancer properties, 3-bromopyruvate (3-BP) is a synthetic derivative of a pyruvate molecule; however, its toxicity in non-malignant cells has not yet been fully elucidated. Therefore, we elected to study the effects of 3-BP on primary hepatocytes in monolayer cultures, permeabilized hepatocytes and isolated mitochondria. After a 1-h treatment with 100 μM 3-BP cell viability of rat hepatocytes was decreased by 30 % as measured by the WST-1 test (p < 0.001); after 3-h exposure to ≥200 μM 3-BP lactate dehydrogenase leakage was increased (p < 0.001). Reactive oxygen species production was increased in the cell cultures after a 1-h treatment at concentrations ≥100 μmol/l (p < 0.01), and caspase 3 activity was increased after a 20-h incubation with 150 μM and 200 μM 3-BP (p < 0.001). This toxic effect of 3-BP was also proved using primary mouse hepatocytes. In isolated mitochondria, 3-BP induced a dose- and time-dependent decrease of mitochondrial membrane potential during a 10-min incubation both with Complex I substrates glutamate + malate or Complex II substrate succinate, although this decrease was more pronounced with the latter. We also measured the effect of 3-BP on respiration of isolated mitochondria. ADP-activated respiration was inhibited by 20 μM 3-BP within 10 min. Similar effects were also found in permeabilized hepatocytes of both species.
Starting Page	363
Ending Page	373
Page Count	11
File Format	PDF
ISSN	0145479X
Journal	Journal of Bioenergetics and Biomembranes
Volume Number	48
Issue Number	4
e-ISSN	15736881
Language	English
Publisher	Springer US
Publisher Date	2016-08-16
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	3-bromopyruvate Toxicity Liver Hepatocyte Mitochondria Bioorganic Chemistry Biochemistry Animal Anatomy / Morphology / Histology Animal Biochemistry Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effect of the antitumoral alkylating agent 3-bromopyruvate on mitochondrial respiration: role of mitochondrially bound hexokinase

3-bromopyruvate: Targets and outcomes

The energy blockers 3-bromopyruvate and lonidamine: effects on bioenergetics of brain mitochondria

Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer

Mitochondria in relation to cancer metastasis: introduction to a mini-review series

Targeting aerobic glycolysis: 3-bromopyruvate as a promising anticancer drug

Caloric restriction influences hydrogen peroxide generation in mitochondrial sub-populations from mouse liver

Role of reactive oxygen species-mediated mitochondrial dysregulation in 3-bromopyruvate induced cell death in hepatoma cells : ROS-mediated cell death by 3-BrPA

New aspects of impaired mitochondrial function in heart failure

Impaired mitochondrial functions contribute to 3-bromopyruvate toxicity in primary rat and mouse hepatocytes

Similar Documents

Effect of the antitumoral alkylating agent 3-bromopyruvate on mitochondrial respiration: role of mitochondrially bound hexokinase

3-bromopyruvate: Targets and outcomes

The energy blockers 3-bromopyruvate and lonidamine: effects on bioenergetics of brain mitochondria

Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer

Mitochondria in relation to cancer metastasis: introduction to a mini-review series

Targeting aerobic glycolysis: 3-bromopyruvate as a promising anticancer drug

Caloric restriction influences hydrogen peroxide generation in mitochondrial sub-populations from mouse liver

Role of reactive oxygen species-mediated mitochondrial dysregulation in 3-bromopyruvate induced cell death in hepatoma cells : ROS-mediated cell death by 3-BrPA

New aspects of impaired mitochondrial function in heart failure

Impaired mitochondrial functions contribute to 3-bromopyruvate toxicity in primary rat and mouse hepatocytes