NDLI: Oxidoreductase activity of chromatophores and purified cytochrome bc $_{ 1 }$ complex from Rhodobacter sphaeroides: a possible role of cardiolipin

Content Provider	Springer Nature Link
Author	Catucci, Lucia Leo, Vincenzo Mila, Francesco Giotta, Livia Vitale, Rita Agostia, Angela Corcelli, Angela
Copyright Year	2012
Abstract	Osmotic shock was used as a tool to obtain cardiolipin (CL) enriched chromatophores of Rhodobacter sphaeroides. After incubation of cells in iso- and hyper-osmotic buffers both chromatophores with a physiological lipid profile (Control) and with an almost doubled amount of CL (CL enriched) were isolated. Spectroscopic properties, reaction centre (RC) and reducible cytochrome (cyt) contents in Control and CL enriched chromatophores were the same. The oxidoreductase activity was found higher for CL enriched than for Control chromatophores, raising from 60 ± 2 to 93 ± 3 mol cyt c s$^{−1}$ (mol total cyt c)$^{−1}$. Antymicin and myxothiazol were tested to prove that oxidoreductase activity thus measured was mainly attributable to the cyt bc $_{ 1 }$ complex. The enzyme was then purified from BH6 strain yielding a partially delipidated and almost inactive cyt bc $_{ 1 }$ complex, although the protein was found to maintain its structural integrity in terms of subunit composition. The ability of CL in restoring the activity of the partially delipidated cyt bc $_{ 1 }$ complex was proved in micellar systems by addition of exogenous CL. Results here reported indicate that CL affects oxidoreductase activity in the bacterium Rhodobacter sphaeroides both in chromatophore and in purified cyt bc $_{ 1 }$ complex.
Starting Page	487
Ending Page	493
Page Count	7
File Format	PDF
ISSN	0145479X
Journal	Journal of Bioenergetics and Biomembranes
Volume Number	44
Issue Number	4
e-ISSN	15736881
Language	English
Publisher	Springer US
Publisher Date	2012-06-26
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rhodobacter sphaeroides Osmotic stress Cardiolipin Oxidoreductase activity Cytochrome bc$_{1}$ complex Animal Anatomy / Morphology / Histology Organic Chemistry Animal Biochemistry Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

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