NDLI: Simultaneous acquisition of 2D and 3D solid-state NMR experiments for sequential assignment of oriented membrane protein samples

Content Provider	Springer Nature Link
Author	Gopinath, T. Mote, Kaustubh R. Veglia, Gianluigi
Copyright Year	2015
Abstract	We present a new method called DAISY (Dual Acquisition orIented ssNMR spectroScopY) for the simultaneous acquisition of 2D and 3D oriented solid-state NMR experiments for membrane proteins reconstituted in mechanically or magnetically aligned lipid bilayers. DAISY utilizes dual acquisition of sine and cosine dipolar or chemical shift coherences and long living $^{15}$N longitudinal polarization to obtain two multi-dimensional spectra, simultaneously. In these new experiments, the first acquisition gives the polarization inversion spin exchange at the magic angle (PISEMA) or heteronuclear correlation (HETCOR) spectra, the second acquisition gives PISEMA-mixing or HETCOR-mixing spectra, where the mixing element enables inter-residue correlations through $^{15}$N–$^{15}$N homonuclear polarization transfer. The analysis of the two 2D spectra (first and second acquisitions) enables one to distinguish $^{15}$N–$^{15}$N inter-residue correlations for sequential assignment of membrane proteins. DAISY can be implemented in 3D experiments that include the polarization inversion spin exchange at magic angle via I spin coherence (PISEMAI) sequence, as we show for the simultaneous acquisition of 3D PISEMAI–HETCOR and 3D PISEMAI–HETCOR-mixing experiments.
Starting Page	53
Ending Page	61
Page Count	9
File Format	PDF
ISSN	09252738
Journal	Journal of Biomolecular NMR
Volume Number	62
Issue Number	1
e-ISSN	15735001
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-03-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Simultaneous acquisition Oriented solid-state NMR Membrane proteins Bicelles Aligned lipid bilayers Biophysics and Biological Physics Biochemistry Spectroscopy/Spectrometry
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Biochemistry

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