NDLI: Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application

Content Provider	Springer Nature Link
Author	Zhao, Yilin Zhang, Zhigang Wang, Jinling Yin, Ping Wang, Yu Yin, Zhenyu Zhou, Jianyin Xu, Gang liu, Yun Deng, Zhigang Zhen, Maochuan Cui, Wugeng Liu, Zhongchen
Copyright Year	2011
Abstract	There is an urgent clinical need of tissue-engineering (TE) vascular grafts, so this study was for developing a fast and simple way of producing TE vascular scaffold. The TE vascular scaffold was prepared with pepsin, DNase and RNase enzymatic decellularization and crosslinked with 0.1, 1, 5% glutaraldehyde (GA), respectively. The samples were underwent analyses of burst pressure; suture strength; cytotoxicity; enzymatic degradation in vitro; degradation in vivo; rehydration; biocompatibilities detected with hematoxylin and eosin (H&E), scan electron microscope, immunohistochemistry both in vivo and in vitro; macrophage infiltration and calcification using Von Kossa staining. After being decellularized the scaffold had a complete removal of cellular components, an intact collagen structure. The burst pressure and suture strength were similar to native artery. 0.1% GA crosslinked scaffold showed less cytotoxicity than 1 and 5% GA groups (P < 0.05) and was resistance to enzymatic degradation in vitro. Once being implanted, 0.1% GA group was resistant to degradation and formed endothelium, smooth muscle and adventitia with few macrophages infiltration. However, there appeared calcification in implants compared with that in native artery. This study demonstrated that DVPs producing methods by enzymatic decellularizing and crosslinking with 0.1% GA could be used for clinical TE vascular graft manufacture.
Starting Page	1407
Ending Page	1417
Page Count	11
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	22
Issue Number	6
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2011-04-28
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Biomaterials Characterization and Evaluation of Materials Metallic Materials Surfaces and Interfaces, Thin Films Ceramics, Glass, Composites, Natural Methods Polymer Sciences
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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