NDLI: Stability of prostaglandin E$_{2}$ (PGE$_{2}$) embedded in poly-d,l-lactide-co-glycolide microspheres: a pre-conditioning approach for tissue engineering applications

Content Provider	Springer Nature Link
Author	Watzer, Bernhard Zehbe, Rolf Halstenberg, Sven James Kirkpatrick, C. Brochhausen, Christoph
Copyright Year	2009
Abstract	Prostaglandin E$_{2}$ (PGE$_{2}$) is involved in angiogenesis, bone repair and cartilage metabolism. Thus, PGE$_{2}$ might represent a suitable signaling molecule in different tissue engineering applications. PGE$_{2}$ also has a short half-life time. Its incorporation into poly-d,l-lactide-co-glycolide (PLGA) microspheres was demonstrated in a previous study. However, the stability of bioactive PGE$_{2}$ in these microspheres is unknown. With an adjusted mass spectrometry assay we investigated the amount of incorporated PGE$_{2}$ and the stability of PGE$_{2}$ in conventional cell culture medium and in PLGA microspheres. The stability of PGE$_{2}$ was closely pH dependent. Strong acidic or basic environments reduced the half-life from 300 h (pH 2.6–4.0) to below 50 h at pH 2.0 or pH 8.8. The half-life of PGE$_{2}$ incorporated into poly-d,l-lactide-co-glycolide increased drastically to 70 days at 37°C and to 300 days at 8°C. Analysis with scanning electron microscopy (SEM) and atomic force microscopy (AFM) demonstrated a distinct nanostructure of the polymeric phase and both nano- and microporosity.
Starting Page	1357
Ending Page	1365
Page Count	9
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	20
Issue Number	6
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2009-01-22
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Surfaces and Interfaces, Thin Films Characterization and Evaluation of Materials Metallic Materials Ceramics, Glass, Composites, Natural Methods Polymer Sciences Biomaterials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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