NDLI: Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results

Content Provider	Springer Nature Link
Author	Bertoldi, S. Farè, S. Denegri, M. Rossi, D. Haugen, H. J. Parolini, O. Tanzi, M. C.
Copyright Year	2009
Abstract	In bone tissue reconstruction, the use of engineered constructs created by mesenchymal stem cells (MSCs) that differentiate and proliferate into 3D porous scaffolds is an appealing alternative to clinical therapies. Human placenta represents a possible source of MSCs, as it is readily available without invasive procedures and because of the phenotypic plasticity of many of the cell types isolated from this tissue. The scaffold considered in this work is a slowly degradable polyurethane foam (EF PU foam), synthesized and characterized for morphology and in vitro interaction with chorion mesenchymal cells (CMCs). These cells were isolated from human term placenta and cultured onto the EF PU foam using two different culture media (EMEM and NH osteogenic differentiation medium). Synthesized EF PU foam showed homogeneous pore size and distribution, with 89% open porosity. In vitro tests showed CMCs scaffold colonization, as confirmed by Scanning Electron Microscopy (SEM) observations and hematoxylin–eosin staining. Alizarin Red staining revealed the presence of a small amount of calcium deposition for the samples treated with the osteogenic differentiation medium. Therefore, the proposed EF PU foam appears to stimulate cell adhesion in vitro, sustaining CMCs growth and differentiation into the osteogenic lineage.
Starting Page	1005
Ending Page	1011
Page Count	7
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	21
Issue Number	3
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2009-12-10
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Surfaces and Interfaces, Thin Films Characterization and Evaluation of Materials Metallic Materials Ceramics, Glass, Composites, Natural Methods Polymer Sciences Biomaterials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Enhanced osteogenic differentiation of cord blood-derived unrestricted somatic stem cells on electrospun nanofibers

Stem cell plasticity, osteogenic differentiation and the third dimension

Characterization of commercial rigid polyurethane foams used as bone analogs for implant testing

Chemico-physical modifications induced by plasma and ozone sterilizations on shape memory polyurethane foams

Osteogenic properties of calcium phosphate ceramics and fibrin glue based composites

Novel method of preparing hydroxyapatite foams

Immobilized MWCNT support osteogenic cell culture

Nanophase hydroxyapatite and poly(lactide-co-glycolide) composites promote human mesenchymal stem cell adhesion and osteogenic differentiation in vitro

Polyurethane/poly(hydroxyethyl methacrylate) semi-interpenetrating polymer networks for biomedical applications

Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results

Similar Documents

Enhanced osteogenic differentiation of cord blood-derived unrestricted somatic stem cells on electrospun nanofibers

Stem cell plasticity, osteogenic differentiation and the third dimension

Characterization of commercial rigid polyurethane foams used as bone analogs for implant testing

Chemico-physical modifications induced by plasma and ozone sterilizations on shape memory polyurethane foams

Osteogenic properties of calcium phosphate ceramics and fibrin glue based composites

Novel method of preparing hydroxyapatite foams

Immobilized MWCNT support osteogenic cell culture

Nanophase hydroxyapatite and poly(lactide-co-glycolide) composites promote human mesenchymal stem cell adhesion and osteogenic differentiation in vitro

Polyurethane/poly(hydroxyethyl methacrylate) semi-interpenetrating polymer networks for biomedical applications

Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results