NDLI: Development of a mini 3D cell culture system using well defined nickel grids for the investigation of cell scaffold interactions

Content Provider	Springer Nature Link
Author	Sun, Tao Smallwood, Rod MacNeil, Sheila
Copyright Year	2009
Abstract	A bioreactor system was developed using a series of fine mesh nickel grids as free standing scaffolds to investigate the behaviours of fibroblasts and keratinocytes in tissue culture. It was found that the mesh size of the suspended grids, but not of the grids that attached to tissue culture surface, had significant influences on cell behaviour and there was a maximum size for fibroblast to span within the defined culture period. Time lapse video microscopy demonstrated fibroblasts cultured on these grids initially migrated onto the struts but then worked together to fill in the voids between struts with a membranous sheet of tissue. In contrast keratinocytes barely migrated from the initial site of cell deposition and when they moved (to a modest extent) it was as an integrated sheet of cells. Similar results were observed when both types of cells were co-cultured in the system.
Starting Page	1483
Ending Page	1493
Page Count	11
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	20
Issue Number	7
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2009-02-20
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Surfaces and Interfaces, Thin Films Characterization and Evaluation of Materials Metallic Materials Ceramics, Glass, Composites, Natural Methods Polymer Sciences Biomaterials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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