NDLI: In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti–6Al

Content Provider	Springer Nature Link
Author	Ponader, Sabine Rosenlehner, Karin Vairaktaris, Eleftherios Wilmowsky, Cornelius Schlegel, Karl A. Neukam, Friedrich W. Schmidt, Cordula D. Schunk, Torsten Hirsch, Andreas Nkenke, Emeka
Copyright Year	2009
Abstract	Layer-by-layer self-assembled films of molecular oligoelectrolytes were used to modify Ti–6Al–4V surfaces in order to test their ability as potential drug delivery system. With regard to medical application the in vitro behavior of the modified material was investigated. The Ti–6Al–4V (6% aluminium, 4% vanadium) material was treated in a layer-by-layer (LbL) process with 2, 4, 6 and 8 layers of molecular oligoelectrolytes 1 and 2 and thereby doped with a fluorescent reporter molecule 2. Human osteoblasts were cultured for a period up to 5 days on the modified material. Ti–6Al–4V surfaces without modification were used as control. In order to investigate the in vitro behavior of the coating as well as the influence of components of the coating on osteoblastic cells, respectively, cell proliferation, differentiation and attachment of hFOB cells were observed by means of cell number, osteoblastic gene expression and fluorescence microscopy. Degradation behavior of the OEM (oligoelectrolyte multilayer film) was examined using optical spectroscopy. Measurement data imply that the layer-by-layer coating was successfully assembled on the Ti surface and endures steam sterilization. The fluorescence signal in cell culture medium increased strictly linear with increasing pre-assembled number of layers on the surface. Proliferation rates of the cells in experimental groups did not differ significantly from each other (P ≥ 0.783). Differentiation pattern was not significantly changed by the coating. The fluorescent reporter component of the film was absorbed by osteoblastic cells and was detected by fluorescence microscopy.
Starting Page	2455
Ending Page	2463
Page Count	9
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	20
Issue Number	12
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2009-07-26
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Surfaces and Interfaces, Thin Films Characterization and Evaluation of Materials Metallic Materials Ceramics, Glass, Composites, Natural Methods Polymer Sciences Biomaterials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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