NDLI: Using liquid crystals for the real-time detection of urease at aqueous/liquid crystal interfaces

Content Provider	Springer Nature Link
Author	Hu, Qiong Zheng Jang, Chang Hyun
Copyright Year	2011
Abstract	In this study, we developed a simple and label-free liquid crystal (LC)-based biosensor for real-time screening of the presence of urease in aqueous solutions. Nematic liquid crystal, 4-cyano-4′-pentylbiphenyl (5CB), when treated with ultraviolet light, showed a bright to dark optical response to the emergence of ammonia in the aqueous phase, indicating a planar to homeotropic alignment of LC. The ammonia-driven optical signal could be ascribed to the orientational transition of 5CB, caused by the deprotonation of 4-cyano-4′-biphenylcarboxylic acid (CBA), which was the main product of UV-treatment and the screening of the electrostatic interactions at the aqueous/LC interface. Due to the high spatial resolution and fast response, this method was successfully used to detect urease belonging to the family of amidohydrolase, which hydrolyzes urea into ammonia and carbon dioxide. A urease concentration as low as 1 nM could be detected and monitored in real-time. Moreover, no signal was detected when a divalent copper ion, which blocks the active sites of urease, was used. This result further confirmed our initial hypothesis that orientational transitions of LC were induced from the enzymatic reaction. The results reported in this article suggest that our method may potentially have clinical utility for the specific detection of Helicobacter pylori. In addition, this ammonia-based LC biosensor may be used for the sensitive detection of other amidohydrolases.
Starting Page	969
Ending Page	975
Page Count	7
File Format	PDF
ISSN	00222461
Journal	Journal of Materials Science
Volume Number	47
Issue Number	2
e-ISSN	15734803
Language	English
Publisher	Springer US
Publisher Date	2011-08-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Continuum Mechanics and Mechanics of Materials Mechanics Polymer Sciences Crystallography Materials Science Characterization and Evaluation of Materials
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Mechanics of Materials Mechanical Engineering Polymers and Plastics

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