NDLI: Specific binding of tubeimoside-2 with proteins in hepatocarcinoma HepG2 cells: investigation by molecular spectroscopy

Content Provider	Springer Nature Link
Author	Yang, Sun Shi Sheng, Sun Ying Yong, Zhao Jun, Fan
Copyright Year	2012
Abstract	In this study, we compared different binding interactions of TBMS2 with proteins both in hepatocarcinoma HepG2 cells and in normal embryo hepatic L02 cells by using fluorescence, absorption, and CD spectroscopy. The fluorescence data revealed that the fluorescence intensity of proteins in the HepG2 and L02 cells decreased in the presence of TBMS2 by 30.79% and 12.01%, respectively. Binding constants and thermodynamic parameters were obtained for systems of TBMS2 with the two kinds of cell proteins. The results indicated that HepG2 cell proteins had a higher TBMS2 binding activity than those in the L02 cells. Analysis of the TBMS2 cytotoxic activities showed that TBMS2 could selectively induce apoptosis of HepG2 cells by binding to them, while its apoptotic effect on L02 cells was relatively weaker.
Starting Page	416
Ending Page	423
Page Count	8
File Format	PDF
ISSN	00219037
Journal	Journal of Applied Spectroscopy
Volume Number	79
Issue Number	3
e-ISSN	15738647
Language	English
Publisher	Springer US
Publisher Date	2012-07-28
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	tubeimoside-2 hepatocarcinoma cells specific binding molecular spectroscopy cytotoxicity Analytical Chemistry Atomic/Molecular Structure and Spectra
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Condensed Matter Physics

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Specific binding of tubeimoside-2 with proteins in hepatocarcinoma HepG2 cells: investigation by molecular spectroscopy