NDLI: Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina

Content Provider	Springer Nature Link
Author	He, Qinghua Qiao, Dairong Zhang, Qinglian Li, Yin Xu, Hui Wei, Liang Gu, Ying Cao, Yi
Copyright Year	2004
Abstract	A nitrate transporter gene, named DsNRT2.1 (GeneBank accession number AY621079), from Dunaliella salina has been cloned by screening a cDNA library, which was constructed with mRNAs from D. salina after 60 min treatment with 5 mM nitrate, with a 342 bp NRT2 cDNA fragment from D. salina as a probe. DsNRT2.1 exhibits sequence similarity to those known nitrate transporters of the NRT2 family. A hydrophobicity blot indicated that DsNRT2.1 belongs to the major facilitator superfamily (MFS). Northern analysis showed that an mRNA species of 1.9 kb can be rapidly induced by NO$^{−}$ $_{3}$, but not by NH$^{+}$ $_{4}$. Northern analysis also showed that NaCl could significantly increase the expression of DsNRT2.1.
Starting Page	395
Ending Page	400
Page Count	6
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	16
Issue Number	5
e-ISSN	15735176
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ecology Hydrobiology Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina