NDLI: Molecular cloning and sequence analysis of methionine adenosyltransferase from the economic seaweed Undaria pinnatifida

Content Provider	Springer Nature Link
Author	Qiao, Kun Li, Shi Guo Li, Hong Yan Tong, Shao Ming Hou, He Sheng
Copyright Year	2012
Abstract	The methionine adenosyltransferase gene (MAT) had been isolated from an economic seaweed Undaria pinnatifida by PCR using degenerate primers. The cDNA was 1,491 bp in length with an open reading frame of 1,194 nucleotides, encoding a deduced protein of 397 amino acids. The protein had a predicted molecular weight of 43.2 kDa, and the isoelectric point was 5.244. The sequence contains a 92 bp 5′-untranslated region (UTR) and a 205 bp 3′-UTR. The methionine adenosyltransferase (MAT) sequence of U. pinnatifida (UpMAT) shared 68–92 % identities with the previous published MAT sequences of other species. Phylogenetic analysis indicated that the phylogenetic relationship of UpMAT with some other seaweeds was closer than with those of higher plants. Under different stress conditions, the relative mRNA expression levels of the MAT of U. pinnatifida (UpMAT) were measured by real-time quantitative PCR, and the results demonstrated that the UpMAT might help to protect the alga against various abiotic stresses.
Starting Page	81
Ending Page	87
Page Count	7
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	25
Issue Number	1
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-05-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Undaria pinnatifida UpMAT Molecular cloning Phaeophyta Real-time quantitative PCR Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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