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| Content Provider | Springer Nature Link |
|---|---|
| Author | Beld, Joris Blatti, Jillian L. Behnke, Craig Mendez, Michael Burkart, Michael D. |
| Copyright Year | 2013 |
| Abstract | The fatty acid synthase (FAS) is a conserved primary metabolic enzyme complex capable of tolerating cross-species engineering of domains for the development of modified and overproduced fatty acids. In eukaryotes, acyl-acyl carrier protein thioesterases (TEs) off-load mature cargo from the acyl carrier protein (ACP), and plants have developed TEs for short/medium-chain fatty acids. We showed that engineering plant TEs into the green microalga Chlamydomonas reinhardtii does not result in the predicted shift in fatty acid profile. Since fatty acid biosynthesis relies on substrate recognition and protein–protein interactions between the ACP and its partner enzymes, we hypothesized that plant TEs and algal ACP do not functionally interact. Phylogenetic analysis revealed major evolutionary differences between FAS enzymes, including TEs and ketoacyl synthases (KSs), in which the former is present only in some species, whereas the latter is present in all, and has a common ancestor. In line with these results, TEs appeared to be selective towards their ACP partners, whereas KSs showed promiscuous behavior across bacterial, plant, and algal species. Based on phylogenetic analyses, in silico docking, in vitro mechanistic cross-linking, and in vivo algal engineering, we propose that phylogeny can predict effective interactions between ACPs and partner enzymes. |
| Starting Page | 1619 |
| Ending Page | 1629 |
| Page Count | 11 |
| File Format | |
| ISSN | 09218971 |
| Journal | Journal of Applied Phycology |
| Volume Number | 26 |
| Issue Number | 4 |
| e-ISSN | 15735176 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2013-11-22 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Acyl carrier protein Evolution Algae Bacteria Fatty acid synthase Chlamydomonas reinhardtii Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Aquatic Science |
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