NDLI: Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

Content Provider	Springer Nature Link
Author	Ale, Marcel Tutor Mikkelsen, Jørn Dalgaard Meyer, Anne S.
Copyright Year	2011
Abstract	Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects. Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl$_{2}$ treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature on the yield of fucose-containing sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive effects, and the acid concentration having a negative effect. The model defined an optimized single-step FCSPs extraction procedure for Sargassum sp. (a brown seaweed). A maximal fucose-containing sulfated polysaccharides yield of ∼7% of the Sargassum sp. dry matter was achieved by the optimal extraction procedure of: 0.03 M HCl, 90°C, 4 h. HPAEC-PAD analysis confirmed that fucose, galactose, and glucuronic acid were the major constituents of the polysaccharides obtained by the optimized method. Lower polysaccharide yield, but relatively higher fucose content was obtained with shorter extraction time. The data also revealed that classical multi-step extraction with acid ≥0.2 M HCl at elevated temperature and extended time had a detrimental effect on the FCSPs yield as this treatment apparently disrupted the structural integrity of the polymer and evidently caused degradation of the carbohydrate chains built up of fucose residues.
Starting Page	715
Ending Page	723
Page Count	9
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	24
Issue Number	4
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fucoidan Sargassum Brown seaweed Fucose Bioactive compound Extraction method Freshwater & Marine Ecology Plant Physiology Plant Sciences Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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