NDLI: Flow cytometry sorting of freshwater phytoplankton

Content Provider	Springer Nature Link
Author	Cellamare, Maria Rolland, Anne Jacquet, Stéphan
Copyright Year	2009
Abstract	We used the flow sorting capacities of a benchtop FACSCalibur flow cytometer to analyze the phytoplankton community of four different aquatic ecosystems. We show that despite the high optical, mechanistic, and hydrodynamic stress for the cells while sorted, most of the targeted populations could be isolated and grew in mixed culture media subsequent to sorting. Forty-five phytoplankton taxa were isolated, including green algae (29 species), cyanobacteria (eight), diatoms (seven), and cryptomonads (one). The isolation success average was high since 80% of the total sorted populations grew successfully and 47% constituted monocultures. It is noteworthy, however, that some groups could not be isolated, as for example colonial cyanobacteria, chrysophytes, euglenophytes, desmids, or dinoflagellates, and some species such as Cryptomonas sp. were very sensitive to the sorting process. It is proposed that flow cytometric analysis of freshwater phytoplankton might be a relevant tool for water managers and could be applied in some specific cases, such as early monitoring of blooming taxa or basic bio-monitorings of key species. The higher isolation average obtained from the flow sorting can also be powerful for the physiological or molecular study of some taxa after their cultivation.
Starting Page	87
Ending Page	100
Page Count	14
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	22
Issue Number	1
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-05-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flow cytometry Sorting Phytoplankton Freshwater Culture Ecology Plant Physiology Freshwater & Marine Ecology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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