NDLI: Autoinduction activity of a conditioned medium obtained from high density cultures of the green alga Scenedesmus subspicatus

Content Provider	Springer Nature Link
Author	Grabski, Krzysztof Tukaj, Zbigniew
Copyright Year	2007
Abstract	Culture filtrates (conditioned medium, CM) containing exudates obtained from cells of Scenedesmus subspicatus grown in batch culture were tested for their autoinduction activity. Undiluted CM completely inhibited the proliferation of cells due to depletion of nitrogen in this medium. When undiluted CM was supplemented with fresh bold basal medium (BBM) medium, enhancement of population growth in a dilution-dependent manner was observed. The most effective was 2-fold diluted CM (CM/2), whereas the growth activity of CM/20 decreased considerably. The proliferation of cells at a very low initial density (10 cells mL$^{−1}$) was induced by the addition of CM/2. Biovolume, dry matter production and oxygen evolution by cells grown in 2-, 5- and 10-fold diluted CM increased markedly in both a concentration- and time-dependent manner. Conditioned medium factor (CMF) was mainly a product of rapidly dividing cells. Preliminary characterisation revealed that CMF is heat-stable, resistant to very low pH, highly hydrophilic (poorly soluble in ethanol or diethyl ether) and diffuses through a 1 kDa dialysis membrane. CM can be stored for long periods due to the time-stable activity of CMF. Depending on the enzyme applied, proteolytic digestion completely (papain, bromelain) or partially (subtilisin) abolished the activity of CM. This effect of CM was enhanced by digestion with trypsin. We suggest that CMF may be a low-molecular-weight peptide(s) or glycopeptide(s).
Starting Page	323
Ending Page	330
Page Count	8
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	20
Issue Number	3
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-10-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Conditioned medium Autoinduction Photosynthesis Scenedesmus Ecology Plant Sciences Plant Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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