NDLI: Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

Content Provider	Springer Nature Link
Author	Mecheri, Barbara D’Epifanio, Alessandra Geracita, Antonio Targon Campana, Patricia Licoccia, Silvia
Copyright Year	2012
Abstract	We fabricated an enzyme fuel cell (EFC) device based on glucose as fuel and glucose oxidase (GOx) as biocatalyst. As a strategy to improve GOx stability, preserving at the same time the enzyme catalytic activity, we propose an immobilization procedure to entrap GOx in a polymer matrix based on Nafion and multiwalled carbon nanotubes. Circular dichroism (CD) spectra were recorded to study changes in the 3D structure of GOx that might be generated by the immobilization procedure. The comparison between the CD features of GOx immobilized and free in solution indicates that the shape of the spectra and position of peaks do not significantly change. The bioelectrocatalytic activity toward glucose oxidation of immobilized GOx was studied by cyclic voltammetry and chronoamperometry experiments. Such electrochemical experiments allow monitoring the rate of GOx-catalyzed glucose oxidation and extrapolating GOx kinetic parameters. Results demonstrate that immobilized GOx has high catalytic efficiency, due the maintaining of regular and well-ordered structure of the immobilized enzyme, as indicated by spectroscopic findings. Once investigated the electrode structure–property relationship, an EFC device was assembled using the GOx-based bioanode, and sulfonated poly ether ether ketone as electrolyte membrane. Polarization and power density curves of the complete EFC device were acquired, demonstrating the suitability of the immobilization strategy and materials to be used in EFCs.
Starting Page	181
Ending Page	190
Page Count	10
File Format	PDF
ISSN	0021891X
Journal	Journal of Applied Electrochemistry
Volume Number	43
Issue Number	2
e-ISSN	15728838
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-10-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Enzyme immobilization Circular dichroism spectroscopy Bioelectrocatalysis Proton exchange membrane Enzyme fuel cell Electrochemistry Physical Chemistry Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Materials Chemistry Chemical Engineering Electrochemistry

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