NDLI: Using an online phycocyanin fluorescence probe for rapid monitoring of cyanobacteria in Macau freshwater reservoir

Content Provider	Springer Nature Link
Author	Kong, Yijun Lou, Inchio Zhang, Yiyong Lou, Chong U. Mok, Kai Meng
Copyright Year	2013
Abstract	Monitoring of cyanobacteria and their toxins are traditionally conducted by cell counting, chlorophyll-a (chl-a) determination and cyanotoxin measurements, respectively. These methods are tedious, costly, time consuming, and insensitive to rapid changes in water quality and cyanobacterial abundance. We have applied and tested an online phycocyanin (PC) fluorescence probe for rapid monitoring of cyanobacteria in the Macau Storage Reservoir (MSR) that is experiencing cyanobacterial blooms. The relationships among cyanobacterial abundance, biovolume, cylindrospermopsin concentration, and PC fluorescence were analyzed using both laboratory and in-the-field studies. The performance of the probe was compared with traditional methods, and its advantages and limitations were assessed in pure and mixed cyanobacterial cultures in the laboratory. The proposed techniques successfully estimated the species including Microcystis and Cylindrospermopsis, two toxic species recently observed in the MSR. During February–November, 2010, the PC probe detected high correlations between PC and cell numbers (R 2 = 0.71). Unlike the chl-a content, which indicates only the total algal biomass, the PC pigment specifically indicates cyanobacteria. These results support the PC parameter as a reliable estimate of cyanobacterial cell number, especially in freshwater bodies where the phytoplankton community and structure are stable. Thus, the PC probe is potentially applicable to online monitoring of cyanobacteria.
Starting Page	33
Ending Page	49
Page Count	17
File Format	PDF
ISSN	00188158
Journal	Hydrobiologia
Volume Number	741
Issue Number	1
e-ISSN	15735117
Language	English
Publisher	Springer International Publishing
Publisher Date	2013-11-29
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phycocyanin fluorescence probe Online measurement Cyanobacteria Freshwater reservoir Freshwater & Marine Ecology Ecology Zoology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fluorescence as a probe for physiological integrity of freshwater cyanobacteria

New insights towards the establishment of phycocyanin concentration thresholds considering species-specific variability of bloom-forming cyanobacteria

Detection of Planktothrix rubescens (Cyanobacteria) associated with microcystin production in a freshwater reservoir

Analysis of cylindrospermopsin- and microcystin-producing genotypes and cyanotoxin concentrations in the Macau storage reservoir

Phycocyanin promoter of Spirulina platensis controlling heterologous expression in cyanobacteria

Factors determining periphytic algae succession in a tropical hypereutrophic reservoir

An update to modern taxonomy (2011) of freshwater planktic heterocytous cyanobacteria

Nuisance cyanobacteria in an urbanized impoundment: interacting internal phosphorus loading, nitrogen metabolism, and polymixis

Comparison of sampling and analytical methods for monitoring of cyanobacteria-dominated surface waters

Using an online phycocyanin fluorescence probe for rapid monitoring of cyanobacteria in Macau freshwater reservoir

Similar Documents

Fluorescence as a probe for physiological integrity of freshwater cyanobacteria

New insights towards the establishment of phycocyanin concentration thresholds considering species-specific variability of bloom-forming cyanobacteria

Detection of Planktothrix rubescens (Cyanobacteria) associated with microcystin production in a freshwater reservoir

Analysis of cylindrospermopsin- and microcystin-producing genotypes and cyanotoxin concentrations in the Macau storage reservoir

Phycocyanin promoter of Spirulina platensis controlling heterologous expression in cyanobacteria

Factors determining periphytic algae succession in a tropical hypereutrophic reservoir

An update to modern taxonomy (2011) of freshwater planktic heterocytous cyanobacteria

Nuisance cyanobacteria in an urbanized impoundment: interacting internal phosphorus loading, nitrogen metabolism, and polymixis

Comparison of sampling and analytical methods for monitoring of cyanobacteria-dominated surface waters

Using an online phycocyanin fluorescence probe for rapid monitoring of cyanobacteria in Macau freshwater reservoir