NDLI: Expression and prognostic role of SKIP in human breast carcinoma

Content Provider	Springer Nature Link
Author	Liu, Xiaobing Ni, Qichao Xu, Junfei Sheng, Chenyi Wang, Qingqing Chen, Jinpeng Yang, Shuyun Wang, Hua
Copyright Year	2013
Abstract	Ski-interacting protein (SKIP) is a nuclear hormone receptor-interacting cofactor, interactions with the proto-oncogene Ski, appears to modulate a number of signalling pathways involved in control of cell proliferation and differentiation, and may play a critical role in oncogenesis. In the present study, to investigate the potential roles of SKIP in breast cancer, expression patterns, interaction and the correlation with clinical/prognostic factors of SKIP and Ki-67 were examined among patients with breast cancer. Immunohistochemistry and Western blot analysis were performed for SKIP in 85 breast carcinoma samples. The data were correlated with clinicopathological features. The univariate and multivariate survival analyses were also performed to determine their prognostic significance. We found that SKIP was over expressed in breast carcinoma as compared with the adjacent normal tissues. High expression of SKIP was positively associated with histological grade (P = 0.01) and Ki-67 (P = 0.004). Univariate analysis showed that SKIP expression was associated with a poor prognosis (P = 0.006). While in vitro, following release of breast cancer cell lines from serum starvation, the expression of SKIP was up-regulated, whereas p27 was down-regulated. In addition, we employed small interfering RNA (siRNA) technique to knock down SKIP expression and observed it effects on MDA-MB-231 cells growth. SKIP depletion by siRNA inhibited cell proliferation, blocked S phase and decreased cyclin A and cyclin B levels. On the basis of these results, we suggested that SKIP overexpression was involved in the pathogenesis of breast cancer, which might serve as a future target for breast cancer.
Starting Page	169
Ending Page	180
Page Count	12
File Format	PDF
ISSN	15672379
Journal	The Histochemical Journal
Volume Number	45
Issue Number	2
e-ISSN	15672387
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	SKIP Human breast cancer Ki-67 Prognosis Cell Biology Biomedicine general Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology Histology

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