NDLI: Genome-wide analysis of gene expression in human embryonic tooth germ

Content Provider	Springer Nature Link
Author	Huang, Zhen Hu, Xuefeng Lin, Chensheng Chen, Suzhu Huang, Feng Zhang, Yanding
Copyright Year	2014
Abstract	While numerous genes that play important regulatory roles during tooth development in mice have been identified, little is known about gene expression profile and their function during human odontogenesis. To unveil expression profile of odontogenic genes in humans, we conducted genome-wide gene expression analysis by microarray assays to analyze differential gene expression between tooth germ and lip tissue from 11-week old human fetuses. We identified 167 genes that are strongly expressed in the cap stage tooth germ as compared to the lip tissue. Among them, 145 genes were further identified by gene ontology enrichment analysis that are highly represented in multiple gene ontology classes, include extracellular components, sequence-specific DNA binding proteins, Wnt-protein binding molecules, system development, organogenesis, and cell differentiation. Sixty-seven genes that are known to be associated with mammalian tooth development and tooth abnormalities were identified. Real-time PCR was further employed to validate microarray data. Moreover, in situ hybridization assay demonstrated tooth type specific expression of ISL1 and BARX1 in the incisor, canine, and molar respectively, consistent with microarray results. Our results represent a set of reliable data that could provide a solid base for future elaboration of molecular mechanisms underlying human tooth development.
Starting Page	609
Ending Page	617
Page Count	9
File Format	PDF
ISSN	15672379
Journal	The Histochemical Journal
Volume Number	45
Issue Number	6
e-ISSN	15672387
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-08-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microarray analysis Gene ontology BINGO Tooth development Human Cell Biology Biomedicine general Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology Histology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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