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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhu, Lianxin Yang, Shuyun He, Song Qiang, Fulin Cai, Jing Liu, Rong Gu, Changjiang Guo, Zengya Wang, Chen Zhang, Wei Zhang, Chunhui Wang, Yingying |
| Copyright Year | 2015 |
| Abstract | Breast cancer is the second leading cause of cancer-related death in women. Previously, evidence suggested that ubiquitin-specific protease 14 (USP14) was associated with various signal transduction pathways and tumourigenesis. In this study, we demonstrate that USP14 is a novel therapeutic target in breast cancer. A Western blot analysis of USP14 was performed using seven breast cancer tissues and paired adjacent normal tissues and showed that the expression of USP14 was increased in the breast cancer tissues. Immunohistochemistry was conducted on formalin-fixed paraffin-embedded sections of breast cancer samples from 100 cases. Using Pearson’s χ2 test, it was demonstrated that USP14 expression was associated with the histological grade, lymph node status and Ki-67 expression in the tumour. The Kaplan–Meier analysis revealed that increased USP14 expression in patients with breast cancer was associated with a poorer prognosis. In in vitro experiments, the highly migratory MDA-MB-231 cells that were treated with USP14-shRNA (shUSP14) exhibited decreased motility using Transwell migration assays. Next, we employed a starvation and re-feeding assay, and the CCK-8 assay demonstrated that USP14 regulated breast cancer cell proliferation. Furthermore, we used flow cytometry to analyse cellular apoptosis following USP14 knockdown. Taken together, our results suggested that USP14 was involved in the progression of breast cancer. |
| Starting Page | 69 |
| Ending Page | 80 |
| Page Count | 12 |
| File Format | |
| ISSN | 15672379 |
| Journal | The Histochemical Journal |
| Volume Number | 47 |
| Issue Number | 1 |
| e-ISSN | 15672387 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2015-12-28 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | USP14 Breast cancer Proliferation Migration Apoptosis Cell Biology Biomedicine general Developmental Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Physiology Histology |
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