NDLI: Molecular cloning and expression analysis of a cytochrome P450 gene in tomato

Content Provider	Springer Nature Link
Author	Li, Zhengguo Hao, Yanwei Yang, Yingwu Deng, Wei
Copyright Year	2010
Abstract	A full-length cytochrome P450 cDNA, CYP71A2, was cloned from tomato (Lycopersicon esculentum Mill.) by RT-PCR and RACE. CYP71A2 (GenBank accession no. GQ370622) encoded a single polypeptide of 495 amino acid residues and shared 46–68% of identity with CYP71A1 which associated with avocado fruit ripening. The polypeptide, which held the conserved domains in all P450s, was classified as CYP71. CYP71A2-GFP fusion protein localised in the endoplasmic reticulum. The expression of CYP71A2 was detected in all the tissues (root, leaf, stem, bud, flower, immature green fruit, mature green fruit, breaker fruit, ripe fruit); however, the CYP71A2 expression was utmost in immature green fruit. During development of fruit, the expression of CYP71A2 reduced rapidly at mature green stage, then gradually increased at breaker and ripening stages. CYP71A2 was regulated by wounding, methyl jasmonate and ethylene. Promoter analysis indicated that CYP71A2 regulatory region had all the specific responding elements to these stresses. This suggested that the role of CYP71A2 is pleiotropic in tomato development and its adaptability to the environment.
Starting Page	297
Ending Page	304
Page Count	8
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	61
Issue Number	3
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-05-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	CYP71A2 Cloning Expression Tomato Fruit Agriculture Plant Physiology Plant Anatomy/Development Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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