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| Content Provider | Springer Nature Link |
|---|---|
| Author | Roussos, P.A. Pontikis, C.A. |
| Copyright Year | 2002 |
| Abstract | Single node explants of 'Koroneiki' olive trees werecultured for one month on a modified Driver-Kuniyuki for Walnut medium, lackinggrowth regulators. The explants were subcultured once a month on a mediumsupplemented with zeatin riboside, 6-(γ-γ-dimethylallylamino)purine,6-benzyladenine or thidiazuron. Zeatin riboside proved to be superior to othercytokinins in inducing shoot proliferation. The combination of olive knotextract at 25 or 50 mg l−1 with cytokininssuppressed shoot proliferation. After two months at the proliferation stage,theexplants were cultured for one week in the dark in 1 ml liquidWoody Plant Medium supplemented with IBA, α-NAA or IBA+α-NAA. Theexplants were then transferred to the same solid medium lacking growthregulators, with a small layer of perlite on the surface. The combination ofthetwo auxins at 1+1 mg l−1 resulted in almost 76%rooting. The combination of olive knot extract at 50 mgl−1 with auxins increased the rooting percentage up toalmost 87%. Artificial infection of explants with the bacteriumPseudomonas savastanoi pv. savastanoiinhibited rhizogenesis, even in the presence of auxins. Rooted explants weresuccessfully acclimatised under a mist system, with the survival rate reachingalmost 75%. |
| Starting Page | 295 |
| Ending Page | 304 |
| Page Count | 10 |
| File Format | |
| ISSN | 01676903 |
| Journal | Plant Growth Regulation |
| Volume Number | 37 |
| Issue Number | 3 |
| e-ISSN | 15735087 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2002-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Plant Physiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Plant Science Agronomy and Crop Science |
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