NDLI: Qualitative determination of bioactive metabolites through Q-TOF LC/MS in different parts and undifferentiated cultures of Ulmus wallichiana Planchon

Content Provider	Springer Nature Link
Author	Kumari, Tejaswita Sharma, Chetan Bajpai, Vikas Kumar, Brijesh Srivastava, Mukesh Arya, K. R.
Copyright Year	2014
Abstract	Ulmus wallichiana is an endangered and folk medicinal plant used for healing fractures in India. Recently, the secondary metabolites isolated and identified from its bark revealed osteogenic activity. Considering the medicinal properties of its bark and the source for its identified and isolated metabolites, the requirement for production of alternative pathways was crucial. This study aimed to determine its bioactive metabolite using Quadrupole time-of-flight liquid chromatography-mass spectroscopy (Q-TOF LC/MS) in different parts of this plant with seasonal and altitudinal variability pertaining with the development of a protocol for induction of callus cultures and biomass production. Plant samples were collected during December–March, April–July and August–November in the year 2010–2012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige and Skoog medium containing (0.25 mg l−1) 2,4-Dichlorophenoxy-acetic acid (2,4-D) + (0.05 mg l−1) Kinetin and (0.25 mg l−1) α-Naphthaleneacetic acid + (0.5 mg l−1) 6-Benzylaminopurine in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites.
Starting Page	331
Ending Page	340
Page Count	10
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	75
Issue Number	1
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ulmus wallichiana Bone healing plant Endemic Uttarakhand Callus and cell suspension cultures Q-TOF LC/MS Plant Sciences Plant Anatomy/Development Plant Physiology Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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