NDLI: In vitro propagation and secondary product production by Merwilla plumbea (Lindl.) Speta

Content Provider	Springer Nature Link
Author	Baskaran, Ponnusamy Ncube, Bhekumthetho Staden, Johannes
Copyright Year	2012
Abstract	Merwilla plumbea is a popular but threatened traditional medicinal plant sold at herbal markets in South Africa. It produces bioactive compounds and contributes to Traditional African Medical systems for the treatment of various diseases. Due to high demand, the plant is recommended for commercial cultivation. We investigated in vitro propagation and secondary compound production in in vitro cultures of M. plumbea, cultivated on Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium supplemented with various plant growth regulators [PGRs: adenine sulphate (Ads), benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), thidiazuron (TDZ), kinetin (Kin) and naphthaleneacetic acid (NAA)] and organic elicitors [casein hydrolysate (CH), glutamine (GM), haemoglobin (HB), mebendazole (MBZ), trimethoprim (TMP), yeast extract (YE) and yeast malt broth (YMB)]. A combination of 20 μM GM, 0.45 μM TDZ and 0.054 μM NAA produced the highest number of adventitious shoots per explant (30.6 shoots/explant) after 12 weeks of culture. The regenerated shoots were rooted and the plantlets successfully acclimatized. The total phenolic, flavonoid, gallotannin and condensed tannin contents were determined. The highest levels of flavonoids (50.97 μg CTE/g in shoots) and gallotannins (99.55 μg GAE/g in shoots) were recorded in combinations of 200 mg l−1 YE, 0.45 μM TDZ and 0.054 μM NAA and 100 mg l−1 YMB, 0.45 μM TDZ and 0.054 μM NAA. These increases were around 3- to 16-fold higher than in naturally-grown plants. This system offers the possibility to use in vitro culture techniques for mass propagation, secondary metabolite and pharmacological studies.
Starting Page	235
Ending Page	245
Page Count	11
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	67
Issue Number	3
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-04-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	In vitro regeneration Medicinal plant Merwilla plumbea Organic elicitors Plant growth regulators compounds Agriculture Plant Physiology Plant Anatomy/Development Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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