NDLI: The Tamarix ferritin gene confers low-iron tolerance in transgenic tobacco

Content Provider	Springer Nature Link
Author	Yao, Wenjing Wang, Shengji Zhou, Boru Jiang, Tingbo
Copyright Year	2016
Abstract	Ferritin is one of the important proteins that respond to various stresses in Tamarix androssowii. We cloned a full-length cDNA sequence of a ferritin gene from T. androssowii, named “TaFer1” (GenBank accession number: AB275873), and overexpressed it in tobacco to examine its functions. The transgenic tobacco plants were confirmed by both northern and western blots, indicating that the TaFer1 gene was expressed in transgenic tobacco plants. Interestingly, the expression of tobacco endogenous NtFer1 gene increased significantly in accordance with TaFer1 gene. Evidence from bacterial two-hybrid experiments demonstrated that there were strong interactions between different ferritin subunits, which suggested that the subunit of TaFer1 participated in the protein complex of ferritin that usually contains 24 subunits. Under low-iron condition, the transgenic tobacco showed a higher tolerance that is manifested in the significant increases of ferric reductase activity, iron concentration, chlorophyll concentration, plant height, and fresh weight than that of non-transgenic plants. The results of the present study suggested that the characterization of Tamarix ferritin gene and the interactions between exogenous and endogenous ferritins may increase our understanding of the molecular functions of the Tamarix ferritin gene and find a solution to prevent iron deficiency.
Starting Page	149
Ending Page	158
Page Count	10
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	80
Issue Number	2
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-01-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tamarix Iron Ferritin Tobacco Protein interaction Accession number AB275873 Plant Sciences Plant Anatomy/Development Plant Physiology Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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