NDLI: The influence of relative humidity on vitrification, growth and morphology of Gypsophila paniculata L.

Content Provider	Springer Nature Link
Author	Gribble, Karleen
Copyright Year	1999
Abstract	A vitrification scoring system was devised that comprised a visual assessment of vitrification in vitro followed by transplanting of plantlets ex vitro and recording of plantlet survival rates. This proved to be a simple method of predicting survival of plantlets ex vitro and demonstrated that vitrification severity is more important than acclimitization procedures in promoting survival ex vitro. Growth of normal plantlets in liquid medium in an environment where plantlets are able to transpire made it clear that lack of transpiration as opposed to high water availability is the primary cause for induction of vitrified growth. Finally, small decreases in relative humidity in vitro were found to be sufficient to increase plantlet transpiration to the extent that vitrification is greatly reduced and survival ex vitro increased. Small differences in relative humidity also influenced plantlet morphology with plantlets grown at a higher relative humidity being larger and consisting of more shoots than plantlets grown at a lower relative humidity. It is suggested that in vitro relative humidity may be manipulated to produce plantlets of a particular morphology and should be measured in tissue culture experiments and included in the materials and methods sections of papers to ensure correct interpretation of results.
Starting Page	181
Ending Page	190
Page Count	10
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	27
Issue Number	3
e-ISSN	15735087
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1999-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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