NDLI: Cloning, characterization, and expression of the BjEXPA1 gene and its promoter region from Brassica juncea L.

Content Provider	Springer Nature Link
Author	Sun, Tao Zhang, Yuxiu Chai, Tuanyao
Copyright Year	2010
Abstract	To explore the regulation of expansin expression under heavy metal stresses, a novel expansin gene, BjEXPA1, and its upstream region were obtained from Indian mustard (Brassica juncea L.). BjEXPA1 encodes a cell wall protein of the α-subgroup of the expansin family. The expression of BjEXPA1 is regulated by exogenous phytohormones, NaCl, and heavy metals. Sequence analysis of its upstream region revealed the presence of several putative cis-acting elements, including phytohormone response, abiotic stress response, and tissue-specific elements. GUS activity under the control of the BjEXPA1 promoter allowed observation of BjEXPA1 spatial and temporal expression patterns. The reporter construct indicated that BjEXPA1 is induced by exogenous gibberellin and auxin, and inhibited by abscisic acid, CdCl2 and PEG6000. Transgenic tobacco seedlings overexpressing BjEXPA1 showed an altered phenotype and enhanced sensitivity to cadmium. Higher H2O2 accumulation and extensive plasma membrane damage were detected in the leaves of transgenic seedlings when exposed to Cd, compared with wild-type plants. Our findings support the conclusion that overexpression of BjEXPA1 in tobacco renders plants susceptible to Cd stress, probably due to disruption of the growth process and/or the breakdown of cell walls.
Starting Page	39
Ending Page	51
Page Count	13
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	64
Issue Number	1
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-10-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Expansin Heavy metal Promoter Growth phenotype Agriculture Plant Anatomy/Development Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of an expansin gene and its ripening-specific promoter fragments from sour cherry (Prunus cerasus L.) cultivars

Molecular cloning and expression of an expansin-like gene in ‘Navel’ orange fruit during postharvest stresses

Effects of 24-epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress

Role of Se(VI) in counteracting oxidative damage in Brassica juncea L. under Cr(VI) stress

Isolation and characterization of 24-Epibrassinolide from Brassica juncea L. and its effects on growth, Ni ion uptake, antioxidant defense of Brassica plants and in vitro cytotoxicity

Intraspecific variability of cadmium tolerance in hydroponically grown Indian mustard (Brassica juncea (L.) Czern.) seedlings

Cloning and expression analysis of an anthocyanidin synthase gene homolog from Brassica juncea

Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea

Characterization of Brassica juncea antioxidant potential under salinity stress

Cloning, characterization, and expression of the BjEXPA1 gene and its promoter region from Brassica juncea L.

Similar Documents

Characterization of an expansin gene and its ripening-specific promoter fragments from sour cherry (Prunus cerasus L.) cultivars

Molecular cloning and expression of an expansin-like gene in ‘Navel’ orange fruit during postharvest stresses

Effects of 24-epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress

Role of Se(VI) in counteracting oxidative damage in Brassica juncea L. under Cr(VI) stress

Isolation and characterization of 24-Epibrassinolide from Brassica juncea L. and its effects on growth, Ni ion uptake, antioxidant defense of Brassica plants and in vitro cytotoxicity

Intraspecific variability of cadmium tolerance in hydroponically grown Indian mustard (Brassica juncea (L.) Czern.) seedlings

Cloning and expression analysis of an anthocyanidin synthase gene homolog from Brassica juncea

Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea

Characterization of Brassica juncea antioxidant potential under salinity stress

Cloning, characterization, and expression of the BjEXPA1 gene and its promoter region from Brassica juncea L.