NDLI: Efficiency of Agrobacterium rhizogenes–mediated root transformation of Parasponia and Trema is temperature dependent

Content Provider	Springer Nature Link
Author	Cao, Qingqin Op den Camp, Rik Seifi Kalhor, Maryam Bisseling, Ton Geurts, Rene
Copyright Year	2012
Abstract	Parasponia trees are the only non-legume species that form nitrogen-fixing root nodules with rhizobium. Based on its taxonomic position in relation to legumes (Fabaceae), it is most likely that both lineages have gained this symbiotic capacity independently. Therefore, Parasponia forms a bridging species to understand the evolutionary constraints underlying this symbiosis. However, absence of key technologies to genetically modify Parasponia seriously impeded studies on these species. We employed Agrobacterium rhizogenes to create composite Parasponia andersonii plants that harbour transgenic roots. Here, we provide an optimized protocol to infect P. andersonii as well as its non-symbiotic sister species Trema tomentosa with A. rhizogenes. We show that the transformation efficiency is temperature dependent. Whereas the optimal growth temperature for both these species is 28 °C, the transformation is most efficient when co-cultivation with A. rhizogenes occurs at 21 °C. Using this optimized protocol up to 80 % transformation efficiency can be obtained. These robust transformation platforms will provide a strong tool to unravel the Parasponia–rhizobium symbiosis.
Starting Page	459
Ending Page	465
Page Count	7
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	68
Issue Number	3
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-07-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Parasponia Trema Agrobacterium transformation Symbiosis Transgenic root Composite plant Agriculture Plant Physiology Plant Sciences Plant Anatomy/Development
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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