NDLI: Isolation and characterization of a GAI/RGA-like gene from Gossypium hirsutum

Content Provider	Springer Nature Link
Author	Liao, Wen bin Ruan, Meng bin Cui, Bai ming Xu, Nan fei Lu, Jia ju Peng, Ming
Copyright Year	2009
Abstract	The aim of the investigation reported here was to assess the role of gibberellin in cotton fiber development. The results of experiments in which the gibberellin (GA) biosynthesis inhibitor paclobutrazol (PAC) was tested on in vitro cultured cotton ovules revealed that GA is critical in promoting cotton fiber development. Plant responses to GA are mediated by DELLA proteins. A cotton nucleotide with high sequence homology to Arabidopsis thaliana GAI (AtGAI) was identified from the GenBank database and analyzed with the BLAST program. The full-length cDNA was cloned from upland cotton (Gossypium hirsutum, Gh) and sequenced. A comparison of the putative protein sequence of this cDNA with all Arabidopsis DELLA proteins indicated that GhRGL is a putative ortholog of AtRGL. Over-expression of this cDNA in Arabidopsis plants resulted in the dwarfed phenotype, and the degrees of dwarfism were related to the expression levels of GhRGL. The deletion of 17 amino acids, including the DELLA domain, resulted in the dominant dwarf phenotype, demonstrating that GhRGL is a functional protein that affects plant growth. Real-time quantitative PCR results showed that GhRGL mRNA is highly expressed in the cotton ovule at the elongation stage, suggesting that GhRGL may play a regulatory role in cotton fiber elongation.
Starting Page	35
Ending Page	45
Page Count	11
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	58
Issue Number	1
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-01-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis Cotton fiber development Cotton (Gossypium hirsutum) DELLA protein Gibberellin Agriculture Plant Physiology Plant Anatomy/Development Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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