NDLI: Characterization of the waxy gene in diploid Triticum L. and Aegilops L. species and its geographic distribution

Content Provider	Springer Nature Link
Author	Li, Wei Fu, Bang Bo Li, Zhe Liu, Ya Xi Pu, Zhi En Qi, Peng Fei Jiang, Qian Tao Chen, Guo Yue Wang, Ji Rui Wei, Yu Ming Zheng, You Liang
Copyright Year	2015
Abstract	Waxy gene plays a key role for amylose synthesis in wheat seeds. The study evaluated the Waxy gene variability in 59 accessions of six diploid Triticum and Aegilops species. The percentages of variable sites, singleton variable sites, and parsimony informative sites were 16.38, 5.59, and 10.79 %, respectively. A total of 22 amino acid changes in the transit peptide and the remaining in the mature protein were observed. Moreover, 17 amino acid changes between Triticum and Aegilops species were also detected. Specially, Gla 14 and Phe 153 was observed in diploid Triticum, compared with Thr 14 and Tyr 153 in diploid Aegilops. Two types of amino acids, Gla 5/Val 5 and Ile 140/Val 140, were identified in T. urartu, as well as Val 22/Phe 22, Thr 52/Lys 52, and Gln 54/delete in A. tauschii. The insertion/deletions (InDels) had high frequency in intron region, but very low in transit peptide and exon region. Neighbour-joining tree showed that 146 sequences from 23 species could be clustered into eight groups with the species characterizations. The Wx-B1 of polyploid Triticum were grouped with A. sharonensis, A. longissima, A. searsii, A. speltoides, while Wx-D1 of T. aestivum and T. spelta together with A. umbellulata, A. markgrafii, A. comosa, and A. tauschii. The Wx-A1 of polyploid Triticum were separated clearly with the diploid species. The Wx-B1 could be divided into two subgroups and maybe had two phylogenetic origins, but most of them were related to A. speltoides. Waxy gene of A. tauschii also had two subgroups, and the sequences from southern Caspian (Mazandaran, Iran) were more closed with Wx-D1. The variability of 5′ Un-Translation Region (UTR) of waxy was stronger than intron and exon region based on genetic distance. Phylogeography analysis showed that geography affected strongly the distribution of all accessions along the north–south axis based on the partial open reading fragment (ORF) of waxy gene, A. speltoides and A. longissima along the west–east axis and north–south axis based on exon, and A. tauschii along the west–east axis based on 5′ UTR, respectively. Our results suggest that diploid Triticum and Aegilops have new waxy gene resources; waxy gene could play a more important role in genetic exploitation, genetic relationship evaluation and phylogeography investigation.
Starting Page	987
Ending Page	1002
Page Count	16
File Format	PDF
ISSN	09259864
Journal	Genetic Resources and Crop Evolution
Volume Number	63
Issue Number	6
e-ISSN	15735109
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-07-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aegilops Molecular characterization Phylogeography Triticum Waxy Plant Sciences Plant Systematics/Taxonomy/Biogeography Plant Genetics & Genomics Plant Physiology Agriculture
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Ecology, Evolution, Behavior and Systematics Agronomy and Crop Science

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Molecular diversity of restriction enzyme sites, Indels and upstream open reading frames (uORFs) of 5′ untransalted regions (UTRs) of Waxy genes in Triticum L. and Aegilops L. species

Characterization of the Wx gene in diploid Aegilops species and its potential use in wheat breeding

Identification of Aegilops L. species and Triticum aestivum L. based on chloroplast DNA

Evaluation of geographic distribution of high-molecular-weight glutenin subunits (HMW-GS) in wild and cultivated Triticum species

Phylogenetic relationships among Triticum L. and Aegilops L. species as genome progenitors of bread wheat based on sequence diversity in trnT-F region of chloroplast DNA

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Distribution and ecological diversity of Aegilops L. in the Greater and Lesser Caucasus Regions of Azerbaijan

Molecular analysis of diversity of diploid Triticum species in Iran using ISSR markers

Genetic diversity of HMW glutenin subunits in diploid, tetraploid and hexaploid Triticum species

Characterization of the waxy gene in diploid Triticum L. and Aegilops L. species and its geographic distribution

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