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| Content Provider | Springer Nature Link |
|---|---|
| Author | Borna, F. Luo, S. Ahmad, N. M. Nazeri, V. Shokrpour, M. Trethowan, R. |
| Copyright Year | 2016 |
| Abstract | Motherwort (Leonurus cardiaca L.) is a medicinal plant indigenous to the Mediterranean regions in Europe and Asia. The objective of this study is to apply inter-primer binding site (iPBS) markers to assess the molecular variation and genetic relationships of 89 genotypes of motherwort to assist the genetic improvement of this species. The genotypes comprised 79 from Iran and 10 collected in Australia and 15 additional accessions of two related species (L. heterophyllus Sweet and L. sibiricus L.) collected in Australia, were also included. PCR of 7 iPBS primers (dominant markers) produced a total of 191 bands ranging from 180 to 4000 bp and the mean PIC for primers ranged from 0.2213 to 0.3206 with a mean value 0.2921. The mean expected heterozygosity (0.134), the mean unbiased expected heterozygosity (0.140) and Shannon’s information index (0.213) indicated a high level of inbreeding among the accessions tested. Ordination and cluster analysis showed that the genetic relationships among all accessions could be separated into three major groups—L. cardiaca, L. heterophyllus and L. sibiricus. However, among the 89 accessions of L. cardiaca, genotypes collected from the same geographic region tended to cluster together thus indicating greater genetic similarity. The Motherwort accessions originating in Iran are highly divergent and possess abundant genetic diversity and clearly provide a basis for selection and breeding. The iPBS PCR-based genome fingerprinting technology used in this study is low-cost and effective in differentiating accessions of motherwort and their related species. |
| Starting Page | 479 |
| Ending Page | 492 |
| Page Count | 14 |
| File Format | |
| ISSN | 09259864 |
| Journal | Genetic Resources and Crop Evolution |
| Volume Number | 64 |
| Issue Number | 3 |
| e-ISSN | 15735109 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2016-03-07 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Genetic diversity Germplasm Leonurus cardiaca L. heterophyllus Motherwort Plant breeding Plant Sciences Plant Systematics/Taxonomy/Biogeography Plant Genetics & Genomics Plant Physiology Agriculture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Ecology, Evolution, Behavior and Systematics Agronomy and Crop Science |
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