NDLI: Monocyte macrophage differentiation in vitro: Fibronectin-dependent upregulation of certain macrophage-specific activities

Content Provider	Springer Nature Link
Author	Sudhakaran, P. R. Radhika, A. Jacob, S. S.
Copyright Year	2006
Abstract	Transendothelial migration of monocytes followed by their differentiation into macrophages involves interaction of monocytes with subendothelial matrix. The influence of extracellular matrix on monocyte–macrophage differentiation was studied using an in vitro model system with human PBMC maintained on different matrix protein substrata. Upregulation of macrophage specific marker activities such as endocytosis of modified proteins, changes in expression of cell surface antigen, and production of matrix metalloproteinases was studied. Cells maintained on Fibronectin (Fn) showed significantly higher rate of endocytosis and production of MMP2 and MMP9 when compared to other matrix protein substrata. Immunoblot analysis, ELISA, and zymography showed that Fn-dependent upregulation of MMPs was blocked by antibodies to α5β1 integrin indicating that the Fn effect was mediated by integrins. The Fn effect on mo–mΦ was blocked by genistein and herbimycin. As monocytes differentiate to macrophages there was an increase in the rate of production of Fn. These results indicate the influence of the microenvironment of the cell, particularly Fn, on mo–mΦ differentiation and integrin-mediated downstream signaling through focal adhesion kinase and Src type tyrosine kinase is involved in this.
Starting Page	49
Ending Page	55
Page Count	7
File Format	PDF
ISSN	02820080
Journal	Glycoconjugate Journal
Volume Number	24
Issue Number	1
e-ISSN	15734986
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-11-18
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Monocyte–macrophage MMPs Fibronectin Integrin signaling Pathology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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