NDLI: Natural hybridization between Phlomis lycia D. Don × P. bourgaei Boiss., (Lamiaceae) revealed by RAPD markers

Content Provider	Springer Nature Link
Author	Yüzbaşıoğlu, Ertuğrul Dadandı, Mehmet Yaşar Özcan, Sebahattin
Copyright Year	2007
Abstract	Randomly Amplified Polymorphic DNA markers (RAPD) were used to assess the hybrid identity of individuals sampled as Phlomis × termessi Davis. Out of 95 primers screened, 11 primers produced reproducible amplification patterns used for discrimination of P. × termessi and their parents. Eleven primers produced 81 bands. Forty two percent of the RAPD bands existed in parents. Of the 54 bands found in P. lycia, 19 were found only in this species and 7 of these were monomorphic. Similarly, of 57 RAPD bands observed in P. bourgaei, 18 were found only in P. bourgaei and 6 of these were monomorphic. Among hybrid individuals, 35 of the 73 markers were monomorphic. Fifteen of these existed in individual parents showing that parents were homozygous for these markers. Of the 35 monomorphic bands observed among hybrid individuals, 5 were present in the samples of one of the parents and completely absent from the samples of the other; therefore, additive inheritance is indicated. Of the 5 additive bands, 1 was inherited from P. bourgaei and 4 were inherited from P. lycia. Among 38 polymorhic markers observed in hybrid individuals, 9 were new and hybrid-specific. Pollen fertility was also investigated. Mean pollen fertility for P. lycia and P. bourgaei was 93% and 97% respectively. However, mean pollen fertility for hybrids was 65% (±10.5).
Starting Page	13
Ending Page	20
Page Count	8
File Format	PDF
ISSN	00166707
Journal	Genetica
Volume Number	133
Issue Number	1
e-ISSN	15736857
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-08-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Natural hybridization Phlomis bourgaei Phlomis lycia Phlomis × termessi RAPDs Microbial Genetics and Genomics Human Genetics Plant Genetics & Genomics Animal Genetics and Genomics Life Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Animal Science and Zoology Insect Science

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