NDLI: Variations on stress defences and metallothionein levels in the Senegal sole, Solea senegalensis, during early larval stages

Content Provider	Springer Nature Link
Author	Solé, M. Potrykus, J. Fernández Díaz, C. Blasco, J.
Copyright Year	2005
Abstract	The biochemical status of antioxidant defences of larvae from the commercial, benthic fish, Solea senegalensis, were studied over a period of 28 days from hatching. The parameters studied were: catalase (EC 1.11.1.6), total glutation peroxidase activity (sum of the selenium dependent and independent forms) (EC 1.6.4.2), DT-diaphorase (EC 1.6.99.2), and as an indication of conjugative detoxifying metabolism, the enzyme glutathione S-transferase (GST; EC 2.5.1.18). Oxidative damage was evaluated by the formation of malondialdehyde (MDA) as an indication of lipid peroxidation (LP). Metallothionein (MT) levels were measured by differential pulse polarography and stress proteins (HSP 70 and HSP 60) were detected by immunoblotting, using commercial antibodies. The presence of catalytic activities was observed from hatching day and tended to increase during larval development. Significant changes were seen in most enzymes at day 3, when the larvae finished their endogenous feeding and started to feed on rotifers. DT-diaphorase activities were similar in both NADH and NADPH-dependent forms and, in turn, were about 10-times lower than their correspondent reductases. LP sharply increased at 19 and 28 days post-hatch (dph), suggesting a saturation of the antioxidant defences during metamorphosis. MT showed high values during the endogenous phase and the lowest value corresponded to day 3, when the egg-yolksac was fully reabsorbed; a steady-state value was reached thereafter. Stress proteins were present at all stages, showing distinctiveness in the molecular weight of HSP60 at hatching day and 1 dph. Quantitatively, both forms were significantly elevated at 3 dph, with their ratio (HSP70/HSP60) decreasing over time.
Starting Page	57
Ending Page	66
Page Count	10
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	30
Issue Number	1
e-ISSN	15735168
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-04-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	antioxidant enzymes fish larvae lipid peroxidation metallothionein ROS Solea senegalensis stress proteins Animal Biochemistry Hydrobiology Zoology Animal Anatomy / Morphology / Histology Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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