NDLI: Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica

Content Provider	Springer Nature Link
Author	Ohta, H. Kagawa, H. Tanaka, H. Okuzawa, K. Iinuma, N. Hirose, K.
Copyright Year	1997
Abstract	Repeated injections of salmon pituitary extract (20 mg per fish per week) induced vitellogenesis in feminized, cultivated Japanese eels (Anguilla japonica). Oocytes were attained at the migratory nucleus stage after 11 or 12 injections. Addition of 17,20β-dihydroxy-4-pregnen-3-one (DHP) into the incubation medium induced germinal vesicle breakdown (GVBD) in the oocytes at the migratory nucleus stage. An injection of DHP (2 µg g-1 BW), given 24h after an injection of salmon pituitary extract (20 mg fish-1), succeeded in inducing maturation and ovulation in females which contained occytes at the migratory nucleus stage. Most fish ovulated 15–18h following the DHP injection. Eggs that were ovulated within 15h after the DHP injection showed high fertility and hatchability, but eggs ovulated 18 or 21h after the DHP injection, showed considerably lower fertility and hatchability. A delay between ovulation and stripping of the eggs rapidly decreased both the fertility and hatchability within 6–9h after ovulation, indicating that artificial fertilization must be carried out immediately after ovulation. Repeated injections of human chorionic gonadotropin (hCG) at a concentration of 1 IU g-1 BW week-1 induced spermatogenesis, spermiation, and the acquisition of potential for sperm motility in cultivated males. Most males spermiated after the fifth or sixth injection of hCG, and the milt weight gradually increased and remained constant (1–2 g) from the 11th to 31th injection. Sperm motility peaked 24h after each weekly injection, and decreased from the 3rd day after the injection. Potassium ions are an essential constituent for the maintenance of motility in the eel spermatozoa. Artificial seminal plasma containing 15.2 mM KCl is applicable as a milt diluent. Using these techniques developed for female and male eels, we have succeeded in obtaining many fertilized eggs from cultivated eels.
Starting Page	163
Ending Page	169
Page Count	7
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	17
Issue Number	1-6
e-ISSN	15735168
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Animal Biochemistry Hydrobiology Zoology Animal Anatomy / Morphology / Histology Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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Occurrence of spontaneous polyploids from the eggs obtained by artificial induction of maturation in the Japanese eel (Anguilla japonica)

Maturational factors as indicators of egg quality in Japanese eel, Anguilla japonica

Biochemical composition of eggsin relation to egg quality in the Japanese eel, Anguilla japonica

Differentiation and development of Leydig cell, and induction of spermatogenesis during testicular differentiation in the Japanese eel, Anguilla japonica

11-ketotestosterone potentiates estrogen-induced vitellogenin production in liver of Japanese eel (Anguilla japonica)

Involvement of androgens and growth hormone in the synthesis of vitellogenin in Japanese eel (Anguilla japonica)

Androgen secretion activity of recombinant follicle-stimulating hormone of Japanese eel, Anguilla japonica in immature and maturing eel testes

The expression and localization of corticotropin-releasing hormone and urotensin I transcripts in the Japanese eel, Anguilla japonica

Functional partition of cells in the gill epithelium of the Japanese eel, Anguilla japonica, and the role of hormones

Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica

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