NDLI: Expression pattern of dmrt4 from olive flounder (Paralichthys olivaceus) in adult gonads and during embryogenesis

Content Provider	Springer Nature Link
Author	Wen, Aiyun You, Feng Tan, Xungang Sun, Peng Ni, Jing Zhang, Yuqing Xu, Dongdong Wu, Zhihao Xu, Yongli Zhang, Peijun
Copyright Year	2008
Abstract	The dmrt (doublesex and mab-3 related transcription factor) gene family comprises several transcription factors that share a conserved DM domain. Dmrt1 is considered to be involved in sexual development, but the precise function of other family members is unclear. In this study, we isolated genomic DNA and cDNA sequences of dmrt4, a member of the dmrt gene family, from olive flounder, Paralichthys olivaceus, through genome walking and real-time reverse transcriptase (RT)-PCR. Sequence analysis indicated that its genomic DNA contains two exons and one intron. A transcriptional factor binding sites prediction program identified a sexual development-related protein, Sox9 (Sry-like HMG box containing 9) in its 5′ promoter. Protein alignment and phylogenetic analysis suggested that flounder Dmrt4 is closely related to tilapia Dmo (DM domain gene in ovary). The expression of dmrt4 in adult flounder was sexually dimorphic, as shown by real-time RT-PCR analysis, with strong expression in the testis but very weak expression in the ovary. Its expression was also strong in the brain and gill, but there was only weak or no expression at all in some of the other tissues tested of both sexes. During embryogenesis, its expression was detected in most developmental stages, although the level of expression was distinctive of the various stages. Whole mount in situ hybridization revealed that the dmrt4 was expressed in the otic placodes, forebrain, telencephalon and olfactory placodes of embryos at different developmental stages. These results will improve our understanding of the possible role of flounder dmrt4 in the development of the gonads, nervous system and sense organs.
Starting Page	421
Ending Page	433
Page Count	13
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	35
Issue Number	3
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-10-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Paralichthys olivaceus dmrt4 Real-time reverse transcriptase-PCR Whole mount in situ hybridization Sexually dimorphic expression Zoology Animal Biochemistry Animal Anatomy / Morphology / Histology Animal Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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