NDLI: Inactivation kinetics of formaldehyde on N-acetyl-β-d-glucosaminidase from Nile tilapia (Oreochromis niloticus)

Content Provider	Springer Nature Link
Author	Zhang, Wei Ni Bai, Ding Ping Lin, Xin Yu Chen, Qing Xi Huang, Xiao Hong Huang, Yi Fan
Copyright Year	2013
Abstract	Formaldehyde is a widely used sanitizer in aquaculture in China, while the appropriate concentration is not available to be used effectively and without damage to tilapia much less to its reproductive function. N-acetyl-β-d-glucosaminidase (EC 3.2.1.52, NAGase), hydrolyzing the oligomers of N-acetyl-β-d-glucosamine into monomer, is proved to be correlated with reproduction of male animals. In this paper, NAGase from spermary of tilapia was chosen as the material to study the effects of formaldehyde on its activity in order to further investigate the effects of formaldehyde use on tilapia reproduction. The results showed the relationship between the residual enzyme activity and the concentration of formaldehyde was concentration dependent, and the IC50 value was estimated to be 3.2 ± 0.1 %. Appropriate concentration of formaldehyde leaded to competitive reversible inhibition on tilapia NAGase. Moreover, formaldehyde could reduce the thermal and pH stability of the enzyme. The inactivation kinetics of formaldehyde on the enzyme was studied using the kinetic method of substrate reaction. The inactivation model was setup, and the rate constants were determined. The results showed that the inactivation of formaldehyde on tilapia NAGase was a slow, reversible reaction with partially residual activity. The results will give some basis to determine the concentration of formaldehyde used in tilapia culture.
Starting Page	561
Ending Page	569
Page Count	9
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	40
Issue Number	2
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-09-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nile tilapia (Oreochromis niloticus) N-acetyl-β-d-glucosaminidase Formaldehyde Activity Reversible inactivation kinetics Freshwater & Marine Ecology Animal Physiology Animal Anatomy / Morphology / Histology Animal Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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