NDLI: The role of leptin in lipid metabolism in fatty degenerated hepatocytes of the grass carp Ctenopharyngodon idellus

Content Provider	Springer Nature Link
Author	Lu, Rong Hua Liang, Xu Fang Wang, Min Zhou, Yi Bai, Xiao Li He, Yan
Copyright Year	2012
Abstract	Leptin (Lep) is a key factor in the regulation of energy homeostasis in mammals, but its role in the fatty degenerated hepatocytes of the grass carp Ctenopharyngodon idellus is still unknown. The aim of our study is to determine the underlying mechanism and possible effects of C. idellus Lep function in lipid metabolism in C. idellus fatty degenerated hepatocytes. Fatty degenerated hepatocytes of C. idellus were established through treatment with media containing 0.1 % lipid emulsion (LE). Hepatic triglycerides had markedly accumulated in the treated hepatocytes 48 h later. Furthermore, we demonstrated that Lep dose dependently promoted the release of glycerol, but not FFA, in fatty degenerated hepatocytes. We also found that Lep affected the expression of key genes related to lipid metabolism at the transcriptional and translational levels. A total of ten genes, including HSL, ATGL, PPARα, PPARβ, UCP1, UCP2, PGC-1α, and CPTIα-1b, were markedly upregulated, while SCD1a and PPARγ were downregulated with Lep treatment. Moreover, the protein levels of HSL and ATGL and the LPL activity also significantly increased. The Lep-induced lipolysis was disrupted by the JAK-STAT inhibitor AG490, suggesting that JAK-STAT signaling pathways were involved in the process of Lep-induced lipolysis. Using the IRS-PI(3)K-specific inhibitor W1628, we found that only the Lep-induced downregulation of PPARγ was reduced. This result indicated that the IRS-PI(3)K signaling pathway was involved in the regulation of the adipogenic gene PPARγ. Overall, our results provided evidence that Lep directly stimulated JAK-STAT signaling-mediated lipolysis and fatty acid β-oxidation gene expression in the fatty degenerated hepatocytes of C. idellus and inhibited the adipogenesis mediated by the IRS-PI(3)K signaling pathway.
Starting Page	1759
Ending Page	1774
Page Count	16
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	38
Issue Number	6
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-07-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Leptin Lipid metabolism Grass carp Ctenopharyngodon idellus Fatty degenerated hepatocytes JAK-STAT IRS-PI(3)K Fatty acid β-oxidation Animal Physiology Zoology Animal Anatomy / Morphology / Histology Animal Biochemistry Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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