NDLI: The dynamics of in vitro 17 β-estradiol secretion by isolated ovarian follicles of the rainbow trout (Oncorhynchus mykiss)

Content Provider	Springer Nature Link
Author	Haddy, J.A. Pankhurst, N.W.
Copyright Year	1998
Abstract	This study investigated the effects of human chorionic gonadotropin (hCG), 17-hydroxyprogesterone (17P) and testosterone (T) on the in vitro production of 17β-estradiol (E2) by isolated ovarian follicles of the rainbow trout (Oncorhynchus mykiss). 17P at 100 ng ml-1, and hCG at 100 IU ml-1 stimulated E2 production relative to controls, whereas lower doses were ineffective. T was the most effective in stimulating E2 production, followed by 17P and hCG respectively. The timecourse of E2 production was investigated for both static culture, and incubations with media replacement, with follicles being exposed to hormone treatment for 30 min, 1 or 3 h, or constantly. E2 production was observed after 30 min, 3 and 3-6 h in response to T, 17P and hCG respectively. Under static culture, E2 levels reached maximal levels in 6 h. Longer incubations resulted in further metabolism of E2 to E2-glucuronide, which resulted in the blurring of treatment effects after 18 h. Incubations with media replacement resulted in higher E2 production than in static culture. The results indicate that a 6 h incubation period is sufficient to produce significant increases in E2 production in response to hCG, 17P and T, and that incubations longer than 12 h result in losses E2 from the incubation media. These findings have implications for the validity of using static cultures to examine the effects of hormone treatment on the activity of steroid converting enzymes in vitro.
Starting Page	267
Ending Page	275
Page Count	9
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	18
Issue Number	3
e-ISSN	15735168
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Animal Biochemistry Hydrobiology Zoology Animal Anatomy / Morphology / Histology Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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