NDLI: Molecular cloning of the cDNA encoding the β subunit of thyrotropin and regulation of its gene expression by thyroid hormones in the goldfish, Carassius auratus

Content Provider	Springer Nature Link
Author	Yoshiura, Y. Sohn, Y.C. Munakata, A. Kobayashi, M. Aida, K.
Copyright Year	1999
Abstract	A cDNA encoding the β subunit of thyrotropin (TSH) was isolated from a goldfish (Carassius auratus) pituitary gland cDNA library. By comparing the sequence with other teleost TSHβs, a signal peptide of 19 amino acids and a mature hormone of 131 amino acids were predicted for goldfish TSHβ subunits. The resulting putative mature hormone of 131 amino acids had well-conserved cysteine positions and a putative N-linked glycosylation site; homology was 51–67% with TSHβs from other teleosts, 38–43% with tetrapod TSHβs, but only 27 and 29% with goldfish GTH-Iβ and -IIβ, respectively. We also examined the effects of thyroid hormones (TH) and thiourea (TU, an inhibitor of TH production) treatments on TSH and GTH subunit gene expressions in the goldfish pituitary gland. After thyroxine (T4) treatment, circulating T4 concentration increased and TSHβ mRNA level decreased. Supressing the amount of circulating T4 and triiodothyronine (T3) by TU treatment increased the TSHβ mRNA level. Moreover, T4 replacement therapy (simultaneous treatment of both TU and T4) caused a high level of circulating T4 and a low level of circulating T3, and a decrease in the TSHβ mRNA level. Thus, changing levels of circulating TH exert a negative feedback on the level of TSH β subunit mRNA in goldfish in vivo. On the other hand, GTH subunit mRNA levels were not affected by changes in the levels of circulating TH.
Starting Page	201
Ending Page	210
Page Count	10
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	21
Issue Number	3
e-ISSN	15735168
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1999-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Animal Biochemistry Hydrobiology Zoology Animal Anatomy / Morphology / Histology Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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