NDLI: Production and characterization of recombinant Manchurian trout thyrotropin

Content Provider	Springer Nature Link
Author	Shin, Jihye Kim, Mi Ae Kobayashi, Makito Sohn, Young Chang
Copyright Year	2013
Abstract	Thyrotropin (thyroid-stimulating hormone, TSH), a heterodimeric glycoprotein hormone produced in the pituitary, stimulates the thyroid gland and release of thyroid hormones. In contrast to a well-known efficacy of recombinant mammalian TSHs, there is no report about the production of teleost recombinant TSH and its biological activity. In this study, we report the production of a single-chain recombinant TSH (mtTSH) of Manchurian trout (Brachymystax lenok), by baculovirus in silkworm (Bombyx mori) larvae. The mtTSH was produced in silkworm larvae and characterized as a form of N-linked glycosylation. The cAMP signaling system in transiently transfected COS-7 cells revealed that the mtTSH was recognized by their cognate receptors, salmon TSHα and TSHβ receptors, but not LH receptor. The thyrotropic potency of the mtTSH was examined by rainbow trout basibranchial tissues containing thyroid follicles. The height of follicle epithelial cells was significantly increased by treatments of mtTSH in vivo and in vitro. In conclusion, the present study suggests that the mtTSH produced by baculovirus–silkworm larvae is a biologically active recombinant TSH.
Starting Page	1353
Ending Page	1363
Page Count	11
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	39
Issue Number	5
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-03-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thyrotropin TSH receptor Recombinant hormone Thyroid follicle Manchurian trout Freshwater & Marine Ecology Animal Physiology Animal Anatomy / Morphology / Histology Animal Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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