NDLI: Use of morphological, molecular markers and cytology to differentiate between closely related Gaura coccinea, G. drummondii for breeding purposes

Content Provider	Springer Nature Link
Author	Pietsch, Grace M. Anderson, Neil O.
Copyright Year	2011
Abstract	Collection of wild species is critical to increase crop germplasm gene pools. Gaura coccinea has been identified as an important source of cold tolerance (winter hardiness) for hybridization with non-cold tolerant G. lindheimeri. Identification of species in the wild is often complicated by diagnostic, morphological characteristics that may not be present at the time of collection. G. drummondii, however, can be misidentified as G. coccinea even when using some diagnostic and quantitative traits. Therefore, additional methods of distinguishing between the species are needed. Fifteen populations of both G. coccinea and G. drummondii collected for potential breeding purposes in Texas, California, and Minnesota were used to identify species-specific molecular markers and morphological characteristics. A dendrogram based on randomly amplified polymorphic DNA (RAPD) markers separated the genotypes into two groups, G. coccinea and G. drummondii. One unique RAPD marker was identified in all G. drummondii genotypes, but absent in G. coccinea. Dendrograms based on morphological data separated genotypes into three distinct groups: G. coccinea, G. drummondii, and G. sp. (393-1). Petal/sepal lengths and leaf width were smaller in G. coccinea, while fruit/stipe lengths were smaller in both G. coccinea and genotype 393-1. Cytological analysis of G. coccinea confirmed that most populations were tetraploid (2n = 4x = 28) with one hexaploid (2n = 6x = 42) genotype from Texas (1000-1). G. drummondii were primarily tetraploid (2n = 4x = 28). Occasional triploids were found. These markers, along with screening for cold tolerance, will allow for accurate identification of G. coccinea genotypes for hybridization with G. lindheimeri.
Starting Page	95
Ending Page	109
Page Count	15
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	183
Issue Number	1
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-08-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Diagnostic traits Onagraceae Polyploidy RAPDs Species identification Plant Physiology Biotechnology Plant Sciences Plant Pathology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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