NDLI: AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion

Content Provider	Springer Nature Link
Author	Huo, Yu Meng Liu, Bing Jiang Yang, Yan Yan Miao, Jun Gao, Li Min Kong, Su Ping Wang, Zhen Bao Kita, Hidemi Wu, Xiong
Copyright Year	2015
Abstract	Selection for maintainer lines using molecular markers that distinguish genotypes at the male restorer-of-fertility (Ms) locus would improve the efficiency of breeding F1 hybrids. Here, we developed a multiplex PCR-based co-dominant marker (AcSKP1) in complete linkage disequilibrium with the Ms locus in onion through allele mining and demonstrated its robustness and utility in marker-assisted selection. F1890, the 5′ flanking sequence of F1527, was obtained from male-fertility-restored line 12–12 by genome walking. S1887, homologous sequence of F1890, was acquired from male-sterile line 118. The coding sequences of F1890 and S1887 were predicted by FGENESH and BLASTP to encode putative S-phase kinase-associated protein 1 (SKP1). These were designated as AcSKP1 and have been deposited in GenBank (KM065384 and KM065385). On the basis of the alignment results for the AcSKP1 gene between F1890 and S1887, four compatible primers (FU898, FD898, SU628, and SD628) were selected to develop a multiplex PCR-based co-dominant marker, AcSKP1, which was established in 25 breeding lines, seven hybrid cultivars, one F2 population, two BC1 populations, and four open-pollinated (OP) populations. Linkage disequilibrium among the Ms locus and AcSKP1 and jnurf05 markers was evaluated using the F2 population. There was no recombination between the Ms locus and AcSKP1, and only 0.049 % recombination between AcSKP1 and jnurf05. Subsequently, the AcSKP1 marker was applied to directly select maintainer individuals from four OP populations with different genetic backgrounds. The results detected by the AcSKP1 marker perfectly predicted the genotypes at the Ms locus in the four OP populations according to the phenotypes of testcross progenies.
Starting Page	711
Ending Page	722
Page Count	12
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	204
Issue Number	3
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-01-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Onion (Allium cepa L.) Multiplex PCR-based marker Restorer-of-fertility (Ms) locus AcSKP1 gene Maintainer line Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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Development of simple PCR-based markers linked to the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.)

Construction of high-resolution linkage map of the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.)

A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions

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Application of the Molecular Marker in Linkage Disequilibrium with Ms, a Restorer-of-fertility Locus, for Improvement of Onion Breeding Efficiency

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AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion

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